DNA damage - 2024 ACF Winter at Central Florida

Question

Detection of the phosphorylated histone protein gamma-H2AX (“gamma-h-two-a-x”) is commonly used to assay for this phenomenon. For 10 points each:

[10m] Name this phenomenon induced by Cas9 during CRISPR editing to trigger either non-homologous end joining or HDR. Thymine dimers are produced in the “direct” form of this general phenomenon.

ANSWER: DNA damage [accept double-strand breaks or DSBs; prompt on damage or breaks or lesions or errors by asking “to what molecule?”]

[10e] Unrepaired DNA damage can cause aberrant “micro” examples of this organelle to form. This organelle dissolves during mitosis and contains a cell’s chromosomes.

ANSWER: nucleus [or nuclei; accept micronucleus or micronuclei]

[10h] DNA damage in micronuclei can result in chromothripsis, in which many of these events catastrophically occur simultaneously in a localized cluster. One type of these events forms in the BCR-ABL protein in CML.

ANSWER: chromosomal rearrangements [prompt on reciprocal translocations, deletions, inversions, or duplications by asking “what more general type of event is that?”; prompt on mutations] (The BCR-ABL1 fusion gene is found on the Philadelphia chromosome, which is formed by a translocation between chromosomes 9 and 22.)

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Summary


2024 ACF Winter at UC Berkeley	2024-11-16	Y	3	20.00	100%	100%	0%
2024 ACF Winter at Clemson	2024-11-16	Y	8	10.00	88%	0%	13%
2024 ACF Winter at Lehigh	2024-11-16	Y	8	10.00	100%	0%	0%
2024 ACF Winter at Northwestern	2024-11-16	Y	9	14.44	100%	44%	0%
2024 ACF Winter at Ohio State	2024-11-16	Y	7	11.43	86%	29%	0%
2024 ACF Winter at Online	2024-11-16	Y	8	10.00	75%	13%	13%
2024 ACF Winter at UBC	2024-11-16	Y	3	16.67	100%	67%	0%
2024 ACF Winter at Central Florida	2024-11-16	Y	5	12.00	100%	20%	0%
2024 ACF Winter at Oxford	2024-11-16	Y	11	10.91	91%	18%	0%
2024 ACF Winter at Brandeis	2024-11-16	Y	10	11.00	100%	10%	0%