DNA damage - 2024 ACF Winter

Question

Detection of the phosphorylated histone protein gamma-H2AX (“gamma-h-two-a-x”) is commonly used to assay for this phenomenon. For 10 points each:

[10m] Name this phenomenon induced by Cas9 during CRISPR editing to trigger either non-homologous end joining or HDR. Thymine dimers are produced in the “direct” form of this general phenomenon.

ANSWER: DNA damage [accept double-strand breaks or DSBs; prompt on damage or breaks or lesions or errors by asking “to what molecule?”]

[10e] Unrepaired DNA damage can cause aberrant “micro” examples of this organelle to form. This organelle dissolves during mitosis and contains a cell’s chromosomes.

ANSWER: nucleus [or nuclei; accept micronucleus or micronuclei]

[10h] DNA damage in micronuclei can result in chromothripsis, in which many of these events catastrophically occur simultaneously in a localized cluster. One type of these events forms in the BCR-ABL protein in CML.

ANSWER: chromosomal rearrangements [prompt on reciprocal translocations, deletions, inversions, or duplications by asking “what more general type of event is that?”; prompt on mutations] (The BCR-ABL1 fusion gene is found on the Philadelphia chromosome, which is formed by a translocation between chromosomes 9 and 22.)

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Summary


2024 ACF Winter at UC Berkeley	2024-11-16	Y	3	20.00	100%	100%	0%
2024 ACF Winter at Clemson	2024-11-16	Y	8	10.00	88%	0%	13%
2024 ACF Winter at Lehigh	2024-11-16	Y	8	10.00	100%	0%	0%
2024 ACF Winter at Northwestern	2024-11-16	Y	9	14.44	100%	44%	0%
2024 ACF Winter at Ohio State	2024-11-16	Y	7	11.43	86%	29%	0%
2024 ACF Winter at Online	2024-11-16	Y	8	10.00	75%	13%	13%
2024 ACF Winter at UBC	2024-11-16	Y	3	16.67	100%	67%	0%
2024 ACF Winter at Central Florida	2024-11-16	Y	5	12.00	100%	20%	0%

Data


Berkeley A	Stanford A	10	10	0	20
Stanford L	Berkeley C	10	10	0	20
Berkeley B	stanford M	10	10	0	20
Alabama A	Tennesse A	0	10	0	10
Auburn A	Emory B	0	10	0	10
Georgia Tech F	Auburn C	0	10	0	10
Georgia Tech D	Clemson A	0	10	0	10
Georgia Tech E	Emory A	0	10	0	10
Auburn B	Georgia A	0	10	10	20
Georgia Tech C	Georgia Tech A	0	0	0	0
Tusculum A	Tennesse B	0	10	0	10
Penn State A	Bard A	0	10	0	10
Rutgers A	Johns Hopkins A	0	10	0	10
Lehigh A	Johns Hopkins B	0	10	0	10
Columbia A	Penn B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Rowan A	Penn State B	0	10	0	10
Johns Hopkins B	Princeton A	0	10	0	10
Rutgers C	Princeton A	0	10	0	10
Purdue B	Purdue A	10	10	0	20
Purdue C	UChicago C	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC A	Purdue D	0	10	0	10
UIUC D	UChicago A	0	10	0	10
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU B	UChicago D	10	10	0	20
WashU C	Indiana A	10	10	0	20
UIUC B	WashU D	0	10	0	10
CWRU B	Kenyon A	0	10	0	10
CWRU D (DII)	Michigan D (DII)	0	10	0	10
Michigan A	Kenyon B (DII)	10	0	0	10
Ohio State B (DII)	Michigan State A	0	10	0	10
Michigan State C (UG)	CWRU C (UG)	0	10	0	10
Ohio State A (UG)	Ohio State C (DII)	0	10	0	10
Michigan State B (UG)	Pitt A	10	10	0	20
Missouri	Arkansas	0	10	0	10
Central Oklahoma	Oregon State	0	10	0	10
Mississippi State	Colorado College	0	0	0	0
Iowa	Ole Miss	0	10	10	20
NYU B	Vassar B	0	0	0	0
Texas A	Texas B	0	10	0	10
WUSTL A	Texas C	0	10	0	10
Vassar A	Texas D	10	10	0	20
UBC A	SFU	10	10	0	20
UW B	UBC B	10	10	0	20
UW A	Alberta	0	10	0	10
UF A	Florida Tech A	0	10	0	10
UCF B	Florida Tech B	0	10	0	10
UF C	UF D	0	10	0	10
UF E	Florida State University A	10	10	0	20
UF B	UF F	0	10	0	10