Question
Detection of the phosphorylated histone protein gamma-H2AX (“gamma-h-two-a-x”) is commonly used to assay for this phenomenon. For 10 points each:
[10m] Name this phenomenon induced by Cas9 during CRISPR editing to trigger either non-homologous end joining or HDR. Thymine dimers are produced in the “direct” form of this general phenomenon.
ANSWER: DNA damage [accept double-strand breaks or DSBs; prompt on damage or breaks or lesions or errors by asking “to what molecule?”]
[10e] Unrepaired DNA damage can cause aberrant “micro” examples of this organelle to form. This organelle dissolves during mitosis and contains a cell’s chromosomes.
ANSWER: nucleus [or nuclei; accept micronucleus or micronuclei]
[10h] DNA damage in micronuclei can result in chromothripsis, in which many of these events catastrophically occur simultaneously in a localized cluster. One type of these events forms in the BCR-ABL protein in CML.
ANSWER: chromosomal rearrangements [prompt on reciprocal translocations, deletions, inversions, or duplications by asking “what more general type of event is that?”; prompt on mutations] (The BCR-ABL1 fusion gene is found on the Philadelphia chromosome, which is formed by a translocation between chromosomes 9 and 22.)
<Biology>
Summary
| 2024 ACF Winter at UC Berkeley | 2024-11-16 | Y | 3 | 20.00 | 100% | 100% | 0% |
| 2024 ACF Winter at Clemson | 2024-11-16 | Y | 8 | 10.00 | 88% | 0% | 13% |
| 2024 ACF Winter at Lehigh | 2024-11-16 | Y | 8 | 10.00 | 100% | 0% | 0% |
| 2024 ACF Winter at Northwestern | 2024-11-16 | Y | 9 | 14.44 | 100% | 44% | 0% |
| 2024 ACF Winter at Ohio State | 2024-11-16 | Y | 7 | 11.43 | 86% | 29% | 0% |
| 2024 ACF Winter at Online | 2024-11-16 | Y | 8 | 10.00 | 75% | 13% | 13% |
| 2024 ACF Winter at UBC | 2024-11-16 | Y | 3 | 16.67 | 100% | 67% | 0% |
| 2024 ACF Winter at Central Florida | 2024-11-16 | Y | 5 | 12.00 | 100% | 20% | 0% |
| 2024 ACF Winter at Oxford | 2024-11-16 | Y | 11 | 10.91 | 91% | 18% | 0% |
Data
| CWRU B | Kenyon A | 0 | 10 | 0 | 10 |
| CWRU D (DII) | Michigan D (DII) | 0 | 10 | 0 | 10 |
| Michigan A | Kenyon B (DII) | 10 | 0 | 0 | 10 |
| Ohio State B (DII) | Michigan State A | 0 | 10 | 0 | 10 |
| Michigan State C (UG) | CWRU C (UG) | 0 | 10 | 0 | 10 |
| Ohio State A (UG) | Ohio State C (DII) | 0 | 10 | 0 | 10 |
| Michigan State B (UG) | Pitt A | 10 | 10 | 0 | 20 |