Assays for measuring the activity of this compound based on BRET and FRET methods include CAMYEL and FlCRhR (“flicker”). By forcing one enzyme to bind to calmodulin, a virulence factor produced by members of the genus Bordetella increases levels of this compound. The enzyme that produces this compound can be stimulated using Forskolin, and is activated by the A subunit of cholera toxin, which increases intracellular (10[1])levels of this compound by over 100-fold. (10[1])Low glucose levels cause this molecule to allosterically bind to CRP as part of a positive regulation mechanism. Phosphodiesterases most commonly act on this compound, which is necessary for the activity of protein kinase A. For 10 points, name this secondary messenger molecule made from ATP by adenylyl (10[1])cyclase. ■END■