This technique’s efficiency is most commonly evaluated with TIDE analysis or with a program whose name is a portmanteau of this technique and “espresso.” Variants of this technique (-5[1])that use a comparatively small protein denoted “12a” are notable for generating overhangs. The H840A and D10A mutations of an enzyme used in this technique can disable that enzyme’s RuvC (“ruv-C”) or HNH domains, thus making it catalytically dead. This technique (10[1])uses a protospacer adjacent to an NGG motif as the target for a guide RNA molecule. The discovery that an endonuclease found in S. pyogenes could be used for this technique won Charpentier and Doudna (-5[1])the Nobel Prize in Chemistry. For 10 points, name this technique that uses the bacterial anti-phage enzyme Cas9 (10[1])to edit genes. ■END■ (10[3])