Question

This technique’s efficiency is most commonly evaluated with TIDE analysis or with a program whose name is a portmanteau of this technique and “espresso.” Variants of this technique (-5[1])that use a comparatively small protein denoted “12a” are notable for generating overhangs. The H840A (-5[1])and D10A mutations of an enzyme used in this technique can disable that enzyme’s RuvC (“ruv-C”) or HNH domains, thus making (10[1])it catalytically dead. (10[1])This technique (10[1])uses a protospacer (-5[1])adjacent to an NGG (10[1])motif (10[1])as the target for a (10[1])guide RNA (10[1])molecule. (10[5])The discovery (10[2])that (10[2])an endonuclease (10[1])found in (10[1])S. pyogenes (10[4])could be used for this technique (-5[2])won Charpentier (10[1])and Doudna (-5[1])the (10[1])Nobel Prize (10[1])in Chemistry. (10[1])For 10 points, name this (10[1])technique that uses the bacterial anti-phage (10[1])enzyme Cas9 (10[4])to edit (10[1])genes. (10[2]0[1]-5[1])■END■ (10[10])

ANSWER: CRISPR-Cas9 gene editing [or Clustered Regularly Interspaced Short Palindromic Repeat editing; accept Cas9 gene editing until “Cas9” is read; accept answers involving other Cas enzymes such as Cas12a until “Cas9” is read; accept CRISPResso; prompt on gene editing or genome editing or genome engineering]
<Biology>
= Average correct buzz position

Buzzes

PlayerTeamOpponentBuzz PositionValue
Danny Han (UG)PennJohn Jay27-5
Jeffrey FungHarvard AHarvard B42-5
Vinu HariharBerkeley CBerkeley B6210
Jacob Martin (UG)Maryland B (UG)Virginia C (UG)6510
Noah ChenColumbia BJohns Hopkins6710
Allan LeeStanford CStanford B70-5
Oscar DespardCambridge ASheffield7410
Kevin ParkClaremont AIowa B7510
Ben Russell JonesEdinburghImperial B8010
Ben LaFondCambridge BOxford B8210
Brian Lai (DII)Virginia B (UG)GWU A (UG)8310
Vedang Singhal (DII)UNC D (DII)Liberty A (Grad)8310
Ryan HumphreyTexas AUW A8310
Dimitris KalafatisTexas A&M BTexas A&M A8310
Bryanna ShaoVanderbilt AMTSU8310
Iris Pardue (UG)KenyonMichigan C8510
Alexander ClarkeDartmouth ADartmouth B8510
Sam MooreDurham AWarwick8610
Davin SivertsonAlabamaGeorgia Tech B8610
Russell NipUBC ARice A8810
Dennis Yang (DII)Michigan BOhio State A9010
Michał GerasimiukStanford ABerkeley A9210
Ivvone Zhou (UG)UNC B (UG)Liberty C (DII)9210
Yashwanth Bajji (DII)Michigan AOhio State B9210
Amogh KulkarniGeodesicTennessee9210
Ted WarnerBristolOxford C98-5
Caleb WestKentuckyHarding98-5
Aisling SkeetDurham BImperial A10010
Tom Doyle (UG)VassarColumbia C102-5
Michael Eng (UG)UNC C (UG)Roanoke College A (DII)10310
Joseph Chambers (DII)Virginia A (UG)UNC A (Grad)10510
Jacob RobertsonOxford AKiel10710
Brian Dudley (UG)William & Mary A (UG)JMU A (UG)11210
Grace EarlyIowa AAppalachian State11810
Yared TadesseCornell ACornell C12010
Cade ReinbergerRIT BCornell B12010
Ethan Rehder (UG)Duke A (UG)JMU B (UG)12010
Arjun Bothra (UG)HaverfordNYU B12010
Caleb Kendrick (Grad)Maryland A (Grad)Maryland C (DII)12210
Bianca Van ZileBinghamtonRIT A12310
Cormac BeirneSorbonneMichigan State A12310
Aswath KaraiMichigan State ASorbonne123-5
Elijah ScheinsonOxford CBristol1230
Andrew ZengStanford BStanford C12410
Annie Goodman (Grad)GWU B (Grad)Liberty B (DII)12410
Albert Zhang (UG)Columbia CVassar12410
Jerry VinokurovJohn JayPenn12410
Jacob Hardin-Bernhardt (DII)NYU ARowan12410
Linus LuuCambridge CKCL12410
Jack ObermanSouth CarolinaGeorgia Tech A12410
Caden HausteinHardingKentucky12410
James McCurleyHarvard BHarvard A12410
Phoebe YaoTufts BTufts A12410