Question
This technique’s efficiency is most commonly evaluated with TIDE analysis or with a program whose name is a portmanteau of this technique and “espresso.” Variants of this technique that use a comparatively small protein denoted “12a” are notable for generating overhangs. The H840A and D10A mutations of an enzyme used in this technique can disable that enzyme’s RuvC (“ruv-C”) or HNH domains, thus making it catalytically dead. This technique uses a protospacer adjacent to an NGG motif as the target for a guide RNA molecule. The discovery that an endonuclease found in S. pyogenes could be used for this technique won Charpentier and Doudna the Nobel Prize in Chemistry. For 10 points, name this technique that uses the bacterial anti-phage enzyme Cas9 to edit genes. ■END■
Summary
2024 ACF Regionals @ Berkeley | 01/27/2024 | Y | 3 | 100% | 0% | 33% | 92.67 |
2024 ACF Regionals @ Cornell | 01/27/2024 | Y | 3 | 100% | 0% | 0% | 121.00 |
2024 ACF Regionals @ JMU | 01/27/2024 | Y | 10 | 100% | 0% | 0% | 100.90 |
2024 ACF Regionals @ Nebraska | 01/27/2024 | Y | 6 | 100% | 0% | 17% | 95.00 |
2024 ACF Regionals @ Ohio State | 01/27/2024 | Y | 3 | 100% | 0% | 0% | 89.00 |
2024 ACF Regionals @ Rutgers | 01/27/2024 | Y | 5 | 100% | 0% | 40% | 111.80 |
2024 ACF Regionals @ Imperial | 01/27/2024 | Y | 8 | 88% | 0% | 13% | 93.29 |
2024 ACF Regionals @ Vanderbilt | 01/27/2024 | Y | 5 | 100% | 0% | 20% | 101.80 |
2024 ACF Regionals @ MIT | 01/27/2024 | Y | 3 | 100% | 0% | 33% | 111.00 |
Buzzes
Player | Team | Opponent | Buzz Position | Value |
---|---|---|---|---|
Danny Han (UG) | Penn | John Jay | 27 | -5 |
Jeffrey Fung | Harvard A | Harvard B | 42 | -5 |
Vinu Harihar | Berkeley C | Berkeley B | 62 | 10 |
Jacob Martin (UG) | Maryland B (UG) | Virginia C (UG) | 65 | 10 |
Noah Chen | Columbia B | Johns Hopkins | 67 | 10 |
Allan Lee | Stanford C | Stanford B | 70 | -5 |
Oscar Despard | Cambridge A | Sheffield | 74 | 10 |
Kevin Park | Claremont A | Iowa B | 75 | 10 |
Ben Russell Jones | Edinburgh | Imperial B | 80 | 10 |
Ben LaFond | Cambridge B | Oxford B | 82 | 10 |
Brian Lai (DII) | Virginia B (UG) | GWU A (UG) | 83 | 10 |
Vedang Singhal (DII) | UNC D (DII) | Liberty A (Grad) | 83 | 10 |
Ryan Humphrey | Texas A | UW A | 83 | 10 |
Dimitris Kalafatis | Texas A&M B | Texas A&M A | 83 | 10 |
Bryanna Shao | Vanderbilt A | MTSU | 83 | 10 |
Iris Pardue (UG) | Kenyon | Michigan C | 85 | 10 |
Alexander Clarke | Dartmouth A | Dartmouth B | 85 | 10 |
Sam Moore | Durham A | Warwick | 86 | 10 |
Davin Sivertson | Alabama | Georgia Tech B | 86 | 10 |
Russell Nip | UBC A | Rice A | 88 | 10 |
Dennis Yang (DII) | Michigan B | Ohio State A | 90 | 10 |
Michał Gerasimiuk | Stanford A | Berkeley A | 92 | 10 |
Ivvone Zhou (UG) | UNC B (UG) | Liberty C (DII) | 92 | 10 |
Yashwanth Bajji (DII) | Michigan A | Ohio State B | 92 | 10 |
Amogh Kulkarni | Geodesic | Tennessee | 92 | 10 |
Ted Warner | Bristol | Oxford C | 98 | -5 |
Caleb West | Kentucky | Harding | 98 | -5 |
Aisling Skeet | Durham B | Imperial A | 100 | 10 |
Tom Doyle (UG) | Vassar | Columbia C | 102 | -5 |
Michael Eng (UG) | UNC C (UG) | Roanoke College A (DII) | 103 | 10 |
Joseph Chambers (DII) | Virginia A (UG) | UNC A (Grad) | 105 | 10 |
Jacob Robertson | Oxford A | Kiel | 107 | 10 |
Brian Dudley (UG) | William & Mary A (UG) | JMU A (UG) | 112 | 10 |
Grace Early | Iowa A | Appalachian State | 118 | 10 |
Yared Tadesse | Cornell A | Cornell C | 120 | 10 |
Cade Reinberger | RIT B | Cornell B | 120 | 10 |
Ethan Rehder (UG) | Duke A (UG) | JMU B (UG) | 120 | 10 |
Arjun Bothra (UG) | Haverford | NYU B | 120 | 10 |
Caleb Kendrick (Grad) | Maryland A (Grad) | Maryland C (DII) | 122 | 10 |
Bianca Van Zile | Binghamton | RIT A | 123 | 10 |
Cormac Beirne | Sorbonne | Michigan State A | 123 | 10 |
Aswath Karai | Michigan State A | Sorbonne | 123 | -5 |
Elijah Scheinson | Oxford C | Bristol | 123 | 0 |
Andrew Zeng | Stanford B | Stanford C | 124 | 10 |
Annie Goodman (Grad) | GWU B (Grad) | Liberty B (DII) | 124 | 10 |
Albert Zhang (UG) | Columbia C | Vassar | 124 | 10 |
Jerry Vinokurov | John Jay | Penn | 124 | 10 |
Jacob Hardin-Bernhardt (DII) | NYU A | Rowan | 124 | 10 |
Linus Luu | Cambridge C | KCL | 124 | 10 |
Jack Oberman | South Carolina | Georgia Tech A | 124 | 10 |
Caden Haustein | Harding | Kentucky | 124 | 10 |
James McCurley | Harvard B | Harvard A | 124 | 10 |
Phoebe Yao | Tufts B | Tufts A | 124 | 10 |