This technique’s efficiency is most commonly evaluated with TIDE analysis or with a program whose name is a portmanteau of this technique and “espresso.” Variants of this technique (-5[1])that use a comparatively small protein denoted “12a” are notable for generating overhangs. The H840A (-5[1])and D10A mutations of an enzyme used in this technique can disable that enzyme’s RuvC (“ruv-C”) or HNH domains, thus making (10[1])it catalytically dead. (10[1])This technique (10[1])uses a protospacer (-5[1])adjacent to an NGG (10[1])motif (10[1])as the target for a (10[1])guide RNA (10[1])molecule. (10[5])The discovery (10[2])that (10[2])an endonuclease (10[1])found in (10[1])S. pyogenes (10[4])could be used for this technique (-5[2])won Charpentier (10[1])and Doudna (-5[1])the (10[1])Nobel Prize (10[1])in Chemistry. (10[1])For 10 points, name this (10[1])technique that uses the bacterial anti-phage (10[1])enzyme Cas9 (10[4])to edit (10[1])genes. (10[2]0[1]-5[1])■END■ (10[10])