Question

This technique’s efficiency is most commonly evaluated with TIDE analysis or with a program whose name is a portmanteau of this technique and “espresso.” Variants of this technique that use a comparatively small protein denoted “12a” are notable for generating overhangs. The H840A and D10A mutations of an enzyme used in this technique can disable that enzyme’s RuvC (“ruv-C”) or HNH domains, thus making it catalytically dead. (10[1])This technique uses a protospacer adjacent to an NGG motif as the target for a guide RNA molecule. (10[2])The discovery that an endonuclease found in S. pyogenes (10[1])could be used for this technique won Charpentier and Doudna the (10[1])Nobel Prize (10[1])in Chemistry. For 10 points, name this (10[1])technique that uses the bacterial anti-phage enzyme Cas9 (10[1])to edit (10[1])genes. ■END■ (10[1])

ANSWER: CRISPR-Cas9 gene editing [or Clustered Regularly Interspaced Short Palindromic Repeat editing; accept Cas9 gene editing until “Cas9” is read; accept answers involving other Cas enzymes such as Cas12a until “Cas9” is read; accept CRISPResso; prompt on gene editing or genome editing or genome engineering]
<Biology>
= Average correct buzz position

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Buzzes

PlayerTeamOpponentBuzz PositionValue
Jacob Martin (UG)Maryland B (UG)Virginia C (UG)6510
Brian Lai (DII)Virginia B (UG)GWU A (UG)8310
Vedang Singhal (DII)UNC D (DII)Liberty A (Grad)8310
Ivvone Zhou (UG)UNC B (UG)Liberty C (DII)9210
Michael Eng (UG)UNC C (UG)Roanoke College A (DII)10310
Joseph Chambers (DII)Virginia A (UG)UNC A (Grad)10510
Brian Dudley (UG)William & Mary A (UG)JMU A (UG)11210
Ethan Rehder (UG)Duke A (UG)JMU B (UG)12010
Caleb Kendrick (Grad)Maryland A (Grad)Maryland C (DII)12210
Annie Goodman (Grad)GWU B (Grad)Liberty B (DII)12410