One variant of this technique uses the presence of an “M-spike” to monitor the progression of monoclonal gammopathies of uncertain significance. Alpha-1, alpha-2, beta, and gamma peaks appear after a large albumin peak in a variant of this technique used to detect globulins. This technique can be applied directly to blood samples in SPEP. A variant of this technique uses a solution composed mostly of tris, glycerol, and beta-mercaptoethanol called Laemmli (“LAM-lee”) buffer. (-5[1])Proteins are denatured with a surfactant before they are forced through a polyacrylamide (-5[1])matrix in a variant of this technique (-5[1])called SDS-PAGE. For 10 points, name this technique in which a current is used to separate analytes into distinct bands on a gel. ■END■ (10[2])