One variant of this technique uses the presence of an “M-spike” (10[1])to monitor the progression of monoclonal gammopathies of uncertain significance. Alpha-1, alpha-2, beta, and gamma peaks appear after a large albumin peak in a variant of this technique used to detect globulins. This technique can be applied directly to blood samples in SPEP. A variant of this technique uses a solution composed mostly of tris, glycerol, (10[1])and beta-mercaptoethanol (10[2])called (10[1])Laemmli (“LAM-lee”) buffer. (10[4]-5[2])Proteins are denatured with a surfactant (10[2])before they are forced through a polyacrylamide (10[2]-5[2])matrix in a (10[1])variant (10[1])of this (10[1])technique (-5[1])called SDS-PAGE. (10[2])For 10 points, (10[1])name (10[1])this technique in which (10[1])a current is used (10[2])to separate (10[5])analytes (10[3]-5[1])into distinct (10[1])bands on a gel. ■END■ (10[7]0[1])