One variant of this technique uses the presence of an “M-spike” to monitor the progression of monoclonal gammopathies of uncertain significance. Alpha-1, alpha-2, beta, and gamma peaks appear after a large albumin peak in a variant of this technique used to detect globulins. This technique can be applied directly to blood samples in SPEP. A variant of this technique uses a solution composed mostly of tris, glycerol, and beta-mercaptoethanol called Laemmli (“LAM-lee”) buffer. (10[1])Proteins are denatured with a surfactant (10[1])before they are forced through a polyacrylamide matrix in a variant of this technique called SDS-PAGE. (10[1])For 10 points, name (10[1])this technique in which a current is used to separate (10[2])analytes (10[1]-5[1])into distinct bands on a gel. ■END■ (10[2]0[1])