Question

One variant of this technique uses the presence of an “M-spike” to monitor the progression of monoclonal gammopathies of uncertain significance. Alpha-1, alpha-2, beta, and gamma peaks appear after a large albumin peak in a variant of this technique used to detect globulins. This technique can be applied directly to blood samples in SPEP. A variant of this technique uses a solution composed mostly of tris, glycerol, (10[1])and beta-mercaptoethanol (10[1])called (10[1])Laemmli (“LAM-lee”) buffer. Proteins are denatured with a surfactant before they are forced through a polyacrylamide (-5[1])matrix in a variant of this technique called SDS-PAGE. (10[1])For 10 points, name this technique in which a current is used to separate analytes into distinct bands on a gel. ■END■ (10[2])

ANSWER: gel electrophoresis [or sodium dodecyl sulfate–polyacrylamide gel electrophoresis or serum protein electrophoresis; accept SDS-PAGE or SPEP until read; prompt on western blotting]
<Biology>
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Buzzes

PlayerTeamOpponentBuzz PositionValue
Ryan HumphreyTexas AIowa A6610
Dimitris KalafatisTexas A&M BUBC A6810
Kevin ParkClaremont AMichigan State A6910
Deepak MuthyalaTexas A&M AAppalachian State84-5
Kathy LiangRice AIowa B9310
Cameron WilliamsAppalachian StateTexas A&M A11510
Isaac OlsonUW ASorbonne11510