Question
Structural information about these molecules is stored in the legacy .pdb file format, which can be used in molecular docking simulations of their interactions. For 10 points each:
[10m] Name these molecules that are designated as “bait” and “prey” in the two-hybrid assay to identify interactions between them. These molecules are often fragmented when sequenced via tandem mass spectrometry.
ANSWER: proteins [accept Protein Data Bank; accept protein–protein interactions; prompt on polypeptides]
[10e] Protein–protein interactions are in part driven by these dipole–dipole interactions between electronegative atoms and the lightest element.
ANSWER: hydrogen bonds [or hydrogen bonding or H-bonds or H-bonding]
[10h] A protein’s interactome can be found by fusing it with the promiscuous ligase BirA, which attaches this molecule to binding partners. Those partners are then purified for mass spec sequencing using this molecule’s high affinity for streptavidin.
ANSWER: biotin [or vitamin B7 or vitamin H; prompt on vitamin B]
<Biology>
Summary
| 2024 ACF Winter at UC Berkeley | 2024-11-16 | Y | 3 | 23.33 | 100% | 67% | 67% |
| 2024 ACF Winter at Clemson | 2024-11-16 | Y | 8 | 12.50 | 88% | 25% | 13% |
| 2024 ACF Winter at Northwestern | 2024-11-16 | Y | 9 | 18.89 | 100% | 67% | 22% |
| 2024 ACF Winter at Ohio State | 2024-11-16 | Y | 7 | 11.43 | 86% | 29% | 0% |
| 2024 ACF Winter at Online | 2024-11-16 | Y | 8 | 15.00 | 100% | 50% | 0% |
| 2024 ACF Winter at UBC | 2024-11-16 | Y | 3 | 16.67 | 100% | 0% | 67% |
| 2024 ACF Winter at Oxford | 2024-11-16 | Y | 11 | 12.73 | 91% | 36% | 0% |
Data
| CWRU A (UG) | CWRU C (UG) | 0 | 10 | 0 | 10 |
| OSU C | Kenyon A | 0 | 10 | 0 | 10 |
| Michigan A | Michigan State A | 10 | 10 | 0 | 20 |
| Michigan B | CWRU D (DII) | 0 | 10 | 0 | 10 |
| Michigan State C (UG) | Michigan C (UG) | 0 | 0 | 0 | 0 |
| Michigan D (DII) | CWRU B (UG) | 0 | 10 | 0 | 10 |
| Ohio State A (UG) | Pitt A | 10 | 10 | 0 | 20 |