Question
This technique can be used to create point mutants in site-directed mutagenesis. For 10 points each:
[10m] Name this molecular biology protocol that amplifies DNA. It uses primers and thermal cycling through melting, annealing, and extension steps.
ANSWER: polymerase chain reaction [or PCR]
[10e] DNA encodes for these biomolecules that form polypeptides. These monomers are the building blocks of proteins.
ANSWER: amino acids [prompt on aa or residues]
[10h] Older methods of site-directed mutagenesis also performed this post-translational modification on the DNA template. Cytosines in CpG islands frequently undergo this modification.
ANSWER: methylation [or methylating; accept answers indicating adding a methyl group or methyl group addition]
<Biology>
Summary
| 2024 ACF Fall at Cornell | fall | Y | 10 | 20.00 | 100% | 80% | 20% |
| 2024 ACF Fall at Ohio State | fall | Y | 8 | 20.00 | 100% | 75% | 25% |
| 2024 ACF Fall at Washington | fall | Y | 1 | 20.00 | 100% | 100% | 0% |
| 2024 ACF Fall at Georgia | fall | Y | 12 | 20.83 | 100% | 67% | 42% |
| 2024 ACF Fall at North Carolina | fall | Y | 9 | 22.22 | 100% | 78% | 44% |
| 2024 ACF Fall at Claremont Colleges | fall | Y | 5 | 28.00 | 100% | 100% | 80% |
| 2024 ACF Fall at Rutgers | fall | Y | 8 | 20.00 | 100% | 75% | 25% |
| 2024 ACF Fall at Illinois | fall | Y | 9 | 20.00 | 100% | 67% | 33% |
Data
| Penn B (DII) | Bard A (UG) | 10 | 10 | 10 | 30 |
| Fordham A | Columbia A (UG) | 0 | 10 | 0 | 10 |
| Columbia B | Maryland A (DII) | 10 | 10 | 0 | 20 |
| NYU A (UG) | Lehigh B (DII) | 10 | 10 | 0 | 20 |
| Penn A (DII) | NYU B | 10 | 10 | 0 | 20 |
| Maryland B (DII) | Princeton A (UG) | 10 | 10 | 10 | 30 |
| Columbia J (DII) | Rutgers A (UG) | 10 | 10 | 0 | 20 |
| Lehigh A (UG) | Rutgers B | 0 | 10 | 0 | 10 |