polymerase chain reaction - 2024 ACF Fall

Question

This technique can be used to create point mutants in site-directed mutagenesis. For 10 points each:

[10m] Name this molecular biology protocol that amplifies DNA. It uses primers and thermal cycling through melting, annealing, and extension steps.

ANSWER: polymerase chain reaction [or PCR]

[10e] DNA encodes for these biomolecules that form polypeptides. These monomers are the building blocks of proteins.

ANSWER: amino acids [prompt on aa or residues]

[10h] Older methods of site-directed mutagenesis also performed this post-translational modification on the DNA template. Cytosines in CpG islands frequently undergo this modification.

ANSWER: methylation [or methylating; accept answers indicating adding a methyl group or methyl group addition]

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Summary


2024 ACF Fall at Cornell	fall	Y	10	20.00	100%	80%	20%
2024 ACF Fall at Ohio State	fall	Y	8	20.00	100%	75%	25%
2024 ACF Fall at Washington	fall	Y	1	20.00	100%	100%	0%
2024 ACF Fall at Georgia	fall	Y	12	20.83	100%	67%	42%
2024 ACF Fall at North Carolina	fall	Y	9	22.22	100%	78%	44%
2024 ACF Fall at Claremont Colleges	fall	Y	5	28.00	100%	100%	80%
2024 ACF Fall at Rutgers	fall	Y	8	20.00	100%	75%	25%
2024 ACF Fall at Illinois	fall	Y	9	20.00	100%	67%	33%

Data


Cornell D	Binghamton A	10	10	10	30
Cornell B	Binghamton B	10	10	0	20
CWRU A (UG)	Jefferson County Scholars (DII)	10	10	0	20
CWRU B (DII)	Ohio State C (DII)	0	10	0	10
Michigan D (UG)	CWRU C (UG)	10	10	0	20
Michigan State B	Miami C (DII)	0	10	0	10
Ohio State A (UG)	Michigan B (UG)	10	10	10	30
Penn B (DII)	Bard A (UG)	10	10	10	30
Fordham A	Columbia A (UG)	0	10	0	10
Columbia B	Maryland A (DII)	10	10	0	20
NYU A (UG)	Lehigh B (DII)	10	10	0	20
Cornell F	Binghamton C	10	10	0	20
Cornell C	RIT B	10	10	10	30
U of Rochester A	Cornell E	10	10	0	20
Cornell G	Cornell A	10	10	0	20
RIT C	ESF	10	10	0	20
RIT A	Syracuse	10	10	0	20
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Penn State	U of Rochester B	0	10	0	10
Tennessee A	Alabama A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
South Carolina A	Auburn A	10	10	0	20
Auburn B	Southern	0	10	0	10
Mississippi State A	Auburn C	10	10	10	30
Clemson B	Belmont	0	10	10	20
Clemson A	Georgia Tech B	0	10	0	10
Georgia A	Furman	10	10	10	30
Emory A	Georgia Tech C	10	10	10	30
Emory Oxford	Tennessee B	10	10	0	20
Georgia Tech D	Vanderbilt A	10	10	0	20
Vanderbilt B	South Carolina B	0	10	10	20
Penn A (DII)	NYU B	10	10	0	20
Maryland B (DII)	Princeton A (UG)	10	10	10	30
Columbia J (DII)	Rutgers A (UG)	10	10	0	20
Lehigh A (UG)	Rutgers B	0	10	0	10
Michigan C	Ohio State B (DII)	10	10	0	20
Miami B (UG)	West Virginia A (UG)	10	10	10	30
Michigan A (UG)	West Virginia B (UG)	10	10	0	20
UW Wrath	UBC A	10	10	0	20
Duke A	Appalachian State B	10	10	10	30
Duke B	UNC D	10	10	0	20
Appalachian State A	James Madison A	10	10	10	30
Liberty A	William & Mary	10	10	0	20
UNC B	Liberty C	10	10	0	20
Liberty B	NC State	0	10	0	10
Tusculum	Virginia	10	10	10	30
Virginia Tech A	VCU	10	10	10	30
Virginia Tech B	James Madison B	0	10	0	10
UCLA D	Claremont B	10	10	0	20
UCLA E	Claremont C	10	10	10	30
Claremont A	UCLA A	10	10	10	30
UCSD	UCLA B	10	10	10	30
UCLA C	USC	10	10	10	30
Chicago A	WashU A	10	10	0	20
Notre Dame C	Chicago B	0	10	0	10
Illinois D	Notre Dame B	0	10	0	10
WashU B	Indiana	10	10	10	30
Purdue A	Iowa A	10	10	10	30
Illinois C	Northwestern B	10	10	0	20
Notre Dame A	Northwestern A	10	10	0	20
Iowa B	Purdue B	10	10	10	30
SIUE	Purdue C	0	10	0	10