This technique can be used to create point mutants in site-directed mutagenesis. For 10 points each:
[10m] Name this molecular biology protocol that amplifies DNA. It uses primers and thermal cycling through melting, annealing, and extension steps.
ANSWER: polymerase chain reaction [or PCR]
[10e] DNA encodes for these biomolecules that form polypeptides. These monomers are the building blocks of proteins.
ANSWER: amino acids [prompt on aa or residues]
[10h] Older methods of site-directed mutagenesis also performed this post-translational modification on the DNA template. Cytosines in CpG islands frequently undergo this modification.
ANSWER: methylation [or methylating; accept answers indicating adding a methyl group or methyl group addition]
<Biology>