Question

An immobilized pH gradient can be used in a two-dimensional form of this technique. Isoelectric focusing in this technique can be subject to cathodic drift. Denaturation of proteins to create (10[1])a uniform negative charge in this technique can be accomplished using detergents like SDS. SYBR (“cyber”) Gold is an intercalating agent used in this technique that is less toxic than traditional dyes. The size of molecules is inversely proportional to the migration rate in this technique. Acrylamide and agarose matrices are used in two forms of this technique. For 10 points, identify this laboratory technique that uses an applied electric field (-5[1])to separate biomolecules on a gel. (10[1])■END■

ANSWER: gel electrophoresis [accept DNA or nucleic acid or protein electrophoresis; accept SDS-PAGE; prompt on DNA separation or Western blot or running a gel]
<Biology>
= Average correct buzz position

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Buzzes

PlayerTeamOpponentBuzz PositionValue
Rachel Fanous (DII)Michigan A (UG) Michigan State A 2910
Malcolm D'Souza (DII)Ohio State B (DII) Jefferson County Scholars (DII)99-5
Beckett Gilmore (DII)Jefferson County Scholars (DII)Ohio State B (DII) 10510

Summary

2024 ACF Fall at Ohio StatefallY2100%0%50%67.00
2024 ACF Fall at WashingtonfallY1100%0%0%49.00
2024 ACF Fall at GeorgiafallY1100%0%100%106.00
2024 ACF Fall at North CarolinafallY3100%0%0%52.67
2024 ACF Fall at IllinoisfallY10100%0%10%51.00