Question

An immobilized pH gradient can be used in a two-dimensional form of this technique. Isoelectric focusing in this technique can be subject to cathodic drift. Denaturation of proteins to create a uniform negative charge in this technique can be accomplished using detergents like SDS. (10[1])SYBR (“cyber”) Gold is an intercalating agent used in (10[1])this technique that is less toxic than traditional dyes. The size of molecules (10[1])is inversely proportional to the migration rate in this technique. Acrylamide and agarose matrices are used in two forms of this technique. For 10 points, identify this laboratory technique that uses an applied electric field to separate biomolecules on a gel. ■END■

ANSWER: gel electrophoresis [accept DNA or nucleic acid or protein electrophoresis; accept SDS-PAGE; prompt on DNA separation or Western blot or running a gel]
<Biology>
= Average correct buzz position

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Buzzes

PlayerTeamOpponentBuzz PositionValue
Malachi LedfordTusculumAppalachian State A4310
Cameron WiliamsAppalachian State AUNC B5110
Ilyas ChoukriVirginia Tech ALiberty A6410

Summary

2024 ACF Fall at Ohio StatefallY2100%0%50%67.00
2024 ACF Fall at WashingtonfallY1100%0%0%49.00
2024 ACF Fall at GeorgiafallY1100%0%100%106.00
2024 ACF Fall at North CarolinafallY3100%0%0%52.67
2024 ACF Fall at IllinoisfallY10100%0%10%51.00