Question
An immobilized pH gradient can be used in a two-dimensional form of this technique. Isoelectric focusing in this technique can be subject to cathodic drift. Denaturation of proteins to create a uniform negative charge in this technique can be accomplished using detergents like SDS. SYBR (“cyber”) Gold is an intercalating agent used in this technique that is less toxic than traditional dyes. The size of molecules is inversely proportional to the migration rate in this technique. Acrylamide and agarose matrices are used in two forms of this technique. For 10 points, identify this laboratory technique that uses an applied electric field to separate biomolecules on a gel. ■END■
ANSWER: gel electrophoresis [accept DNA or nucleic acid or protein electrophoresis; accept SDS-PAGE; prompt on DNA separation or Western blot or running a gel]
<Biology>
= Average correct buzz position
Summary
2024 ACF Fall at Ohio State | fall | Y | 2 | 100% | 0% | 50% | 67.00 |
2024 ACF Fall at Washington | fall | Y | 1 | 100% | 0% | 0% | 49.00 |
2024 ACF Fall at Georgia | fall | Y | 1 | 100% | 0% | 100% | 106.00 |
2024 ACF Fall at North Carolina | fall | Y | 3 | 100% | 0% | 0% | 52.67 |
2024 ACF Fall at Illinois | fall | Y | 10 | 100% | 0% | 10% | 51.00 |
Buzzes
Player | Team | Opponent | Buzz Position | Value |
---|---|---|---|---|
Matthew Westley | Purdue A | Notre Dame A | 13 | -5 |
Ishaan Singh | Purdue B | Chicago A | 13 | 10 |
Grace Early | Iowa A | Illinois A | 25 | 10 |
Rachel Fanous (DII) | Michigan A (UG) | Michigan State A | 29 | 10 |
Samay Math | WashU B | Purdue C | 36 | 10 |
Rohan Krishnamoorthi | WashU A | Indiana | 40 | 10 |
Patrick Boyle | Notre Dame B | Notre Dame C | 40 | 10 |
Malachi Ledford | Tusculum | Appalachian State A | 43 | 10 |
Russell Nip | UBC A | UBC B | 49 | 10 |
Cameron Wiliams | Appalachian State A | UNC B | 51 | 10 |
Arthur Zhang | Northwestern A | Illinois D | 53 | 10 |
Ilyas Choukri | Virginia Tech A | Liberty A | 64 | 10 |
Azura Gollamudi | Northwestern B | Illinois B | 64 | 10 |
Abigail Zimmerman | Iowa B | Illinois C | 64 | 10 |
Emmett Cho | Chicago B | SIUE | 73 | 10 |
Jeffery Xu | Georgia Tech D | Auburn A | 74 | -5 |
Malcolm D'Souza (DII) | Ohio State B (DII) | Jefferson County Scholars (DII) | 99 | -5 |
Maya Kvaratskhelia | Notre Dame A | Purdue A | 102 | 10 |
Beckett Gilmore (DII) | Jefferson County Scholars (DII) | Ohio State B (DII) | 105 | 10 |
Henry Bylinowski | Auburn A | Georgia Tech D | 106 | 10 |