Question

An immobilized pH gradient can be used in a two-dimensional form of this technique. (10[1]-5[1])Isoelectric focusing in this technique can be subject to cathodic drift. Denaturation (10[1])of proteins to create (10[1])a uniform negative charge in this technique (10[1])can be accomplished using (10[2])detergents like SDS. (10[1])SYBR (“cyber”) Gold is an intercalating agent (10[1])used in (10[1])this technique (10[1])that is less toxic than traditional dyes. The size of molecules (10[3])is inversely proportional to the migration rate in this (10[1])technique. (-5[1])Acrylamide and agarose matrices are used in two forms of this technique. For 10 points, identify this laboratory technique that uses an applied electric field (-5[1])to separate biomolecules (10[1])on a gel. (10[1])■END■ (10[1])

ANSWER: gel electrophoresis [accept DNA or nucleic acid or protein electrophoresis; accept SDS-PAGE; prompt on DNA separation or Western blot or running a gel]
<Biology>
= Average correct buzz position

Summary

2024 ACF Fall at Ohio StatefallY2100%0%50%67.00
2024 ACF Fall at WashingtonfallY1100%0%0%49.00
2024 ACF Fall at GeorgiafallY1100%0%100%106.00
2024 ACF Fall at North CarolinafallY3100%0%0%52.67
2024 ACF Fall at IllinoisfallY10100%0%10%51.00

Buzzes

PlayerTeamOpponentBuzz PositionValue
Matthew WestleyPurdue ANotre Dame A13-5
Ishaan SinghPurdue BChicago A1310
Grace EarlyIowa AIllinois A2510
Rachel Fanous (DII)Michigan A (UG) Michigan State A 2910
Samay MathWashU BPurdue C3610
Rohan KrishnamoorthiWashU AIndiana4010
Patrick BoyleNotre Dame BNotre Dame C4010
Malachi LedfordTusculumAppalachian State A4310
Russell NipUBC AUBC B4910
Cameron WiliamsAppalachian State AUNC B5110
Arthur ZhangNorthwestern AIllinois D5310
Ilyas ChoukriVirginia Tech ALiberty A6410
Azura GollamudiNorthwestern BIllinois B6410
Abigail ZimmermanIowa BIllinois C6410
Emmett ChoChicago BSIUE7310
Jeffery XuGeorgia Tech DAuburn A74-5
Malcolm D'Souza (DII)Ohio State B (DII) Jefferson County Scholars (DII)99-5
Maya KvaratskheliaNotre Dame APurdue A10210
Beckett Gilmore (DII)Jefferson County Scholars (DII)Ohio State B (DII) 10510
Henry BylinowskiAuburn AGeorgia Tech D10610