Question
The 10x genomics Multiome platform allows simultaneous performance of this technique and RNA sequencing. For 10 points each:
[10h] Name this sequencing technique that measures genome-wide chromatin accessibility. During this technique, Tn5 transposase enzyme cuts and inserts sequencing adapters in accessible regions.
ANSWER: ATAC-seq [or ATAC sequencing; or assay for transposase-accessible chromatin with sequencing]
[10m] The aforementioned 10x genomics platform is used for single-cell sequencing, which uses microfluidics technology to capture individual cells in these spherical beads of liquid.
ANSWER: droplets [prompt on drops; prompt on emulsions; prompt on compartments; prompt on micelles]
[10e] Chromatin accessibility is primarily governed by DNA being wrapped around these charged proteins. Eight of these proteins make up the nucleosome.
ANSWER: histone
<Paul Lee , Science - Biology - Mixed/Any>
Summary
| 2023 NASAT | 06/17/2023 | Y | 9 | 13.33 | 78% | 56% | 0% |
Data
| Maryland Red | California | 0 | 10 | 10 | 20 |
| Illinois Blue | New Jersey A | 0 | 10 | 10 | 20 |
| Illinois White | Illinois Orange | 0 | 0 | 10 | 10 |
| Kentucky A | Missouri A | 0 | 10 | 10 | 20 |
| Kentucky B | New Jersey B | 0 | 0 | 10 | 10 |
| Arkansas | Liberia | 0 | 0 | 0 | 0 |
| Missouri B | Maryland Gold | 0 | 0 | 10 | 10 |
| Ohio | Asia A | 0 | 10 | 10 | 20 |
| Pennsylvania | Asia B | 0 | 10 | 0 | 10 |