The 10x genomics Multiome platform allows simultaneous performance of this technique and RNA sequencing. For 10 points each:
[10h] Name this sequencing technique that measures genome-wide chromatin accessibility. During this technique, Tn5 transposase enzyme cuts and inserts sequencing adapters in accessible regions.
ANSWER: ATAC-seq [or ATAC sequencing; or assay for transposase-accessible chromatin with sequencing]
[10m] The aforementioned 10x genomics platform is used for single-cell sequencing, which uses microfluidics technology to capture individual cells in these spherical beads of liquid.
ANSWER: droplets [prompt on drops; prompt on emulsions; prompt on compartments; prompt on micelles]
[10e] Chromatin accessibility is primarily governed by DNA being wrapped around these charged proteins. Eight of these proteins make up the nucleosome.
ANSWER: histone
<Paul Lee , Science - Biology - Mixed/Any>