This organism’s TOP10 strain is genetically engineered for high transformation efficiency. For 10 points each:
[10e] Name this bacterium that can be grown on agar plates. Along with salmonella, this organism causes food poisoning, commonly seen with raw lettuce.
ANSWER: E. coli [or Escherichia coli]
[10m] Desired genetic material can be introduced into E. coli cells using these DNA molecules that contain an origin of replication. Restriction enzymes allow for the insertion of genes of interest into these molecules.
ANSWER: plasmids
[10h] The KRX strain of E. coli is incapable of conjugation as it is negative for this specific plasmid. The proteases OmpF and OmpT are encoded on plasmid present in Hfr cells.
ANSWER: F plasmid [accept sex plasmid or sex factor]
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