E. coli - 2025 ACF Winter - Buzzpoints App

Question

This organism’s TOP10 strain is genetically engineered for high transformation efficiency. For 10 points each:

[10e] Name this bacterium that can be grown on agar plates. Along with salmonella, this organism causes food poisoning, commonly seen with raw lettuce.

ANSWER: E. coli [or Escherichia coli]

[10m] Desired genetic material can be introduced into E. coli cells using these DNA molecules that contain an origin of replication. Restriction enzymes allow for the insertion of genes of interest into these molecules.

ANSWER: plasmids

[10h] The KRX strain of E. coli is incapable of conjugation as it is negative for this specific plasmid. The proteases OmpF and OmpT are encoded on plasmid present in Hfr cells.

ANSWER: F plasmid [accept sex plasmid or sex factor]

<Editors, Biology>

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Summary


Canada	Main	Y	6	18.33	100%	83%	0%
Florida	Main	Y	13	16.15	100%	54%	8%
Midwest	Main	Y	8	18.75	100%	75%	13%
Northeast	Main	Y	5	22.00	100%	100%	20%
Pacific Northwest	Main	Y	10	24.00	100%	90%	50%
South Central	Main	Y	5	14.00	100%	40%	0%
Upper Mid-Atlantic	Main	Y	8	17.50	100%	75%	0%
Upstate NY	Main	Y	8	16.25	88%	38%	38%
2025 ACF Nationals	2025-08-09	Y	3	20.00	100%	67%	33%

Data


UCLA A	Claremont Pitzer	10	10	0	20
UCLA C	UCI	10	10	0	20
Claremont Scripps	UCLA B	10	10	0	20
UCSD What Hath God Wrought	UCLA D	10	10	0	20
USC	UCSB	10	10	0	20
UCSD Vices and Virtues	UCSD Two Running Violet V Forms	10	0	0	10
McGill B	Carleton	10	0	0	10
Queen's A	McGill C	10	10	0	20
McMaster B	Guelph B	10	10	10	30
Toronto C	Ottawa	10	0	0	10
Western Ontario	Queen's B	10	0	0	10
Toronto A	Guelph A	10	10	0	20
McMaster A	Toronto D	10	10	0	20
Waterloo	Toronto E	10	0	0	10
Toronto G	Toronto Metropolitan	10	0	0	10
McMaster C	Toronto I	10	10	0	20
Toronto F	Toronto J	10	0	0	10
Toronto H	Toronto K	10	10	0	20
Toronto B	McGill A	10	10	0	20
Liberty C	Clemson A	10	0	0	10
Virginia B	James Madison	10	10	0	20
Liberty A	North Carolina B	10	10	0	20
South Carolina A	Liberty B	10	10	10	30
Clemson B	South Carolina B	10	0	0	10
Virginia Tech B	Tusculum	10	10	0	20
Virginia Tech A	North Carolina C	10	10	0	20
NC State	North Carolina A	10	10	0	20
Rowan A	Columbia A	10	10	10	30
Columbia B	Lehigh A	10	10	0	20
Columbia C	Vassar A	10	10	0	20
Rutgers B	Princeton A	10	10	0	20
Rutgers C	Lehigh B	10	10	0	20
Kentucky	Chicago A	10	10	0	20
Chicago B	Notre Dame B	10	10	10	30
Illinois C	Chicago D	10	10	0	20
Michigan B	Illinois A	10	10	10	30
Notre Dame C	Indiana B	10	10	0	20
Notre Dame A	Louisville A	10	10	0	20
Louisville B	Chicago C	10	10	10	30
Michigan C	Purdue C	10	10	10	30
Michigan D	Illinois B	10	0	0	10
Purdue A	Notre Dame D	10	10	10	30
Macalester A	Iowa State	10	10	0	20
St. Thomas	Minnesota B	10	0	0	10
Carleton	Nebraska	10	10	0	20
Minnesota C	Wisconsin	10	0	0	10
UCO	Arizona State A	10	0	0	10
Arizona State B	Tulsa	10	0	0	10
Ohio	Iowa	10	10	0	20
Northwestern B	McGill D	10	10	0	20
Northwestern A	Texas B	10	10	0	20
UTD A	Rose State	10	10	0	20
Texas A	Alberta B	10	10	0	20
Northwestern C	UTD B	10	10	0	20
Macalester B	Minnesota A	10	0	0	10
Alabama B	Auburn B	10	0	0	10
Georgia Tech A	Auburn A	10	10	10	30
Bruin A	Georgia Tech C	0	0	0	0
Georgia Tech D	Georgia Tech B	10	10	0	20
Alabama A	Huntsville A	10	0	10	20
Mississippi State A	Huntsville B	10	0	0	10
UAB A	Sewanee A	10	10	10	30
Vanderbilt A	Vanderbilt B	10	0	0	10
Southampton B	Sheffield A	10	10	0	20
Oxford B	Cambridge D	10	0	0	10
Warwick A	Durham A	10	10	10	30