The first step of one form of glycosylation begins with the insertion of dolichol (“daw-lih-CALL”) phosphate into the membrane of this organelle. For 10 points each:
[10e] Name this organelle that processes and packages peptides to the Golgi apparatus. Ribosomes stud this organelle’s rough form.
ANSWER: endoplasmic reticulum [or ER; accept rough endoplasmic reticulum or RER]
[10m] After insertion into the ER membrane, enzymes attach NAG and this molecule onto dolichol to create an oligosaccharide. Proteins tagged with a derivative of this molecule are directed to a hydrolase-rich structure.
ANSWER: mannose [accept mannose-6-phosphate]
[10h] After being translocated to the lumen side, a transferase attaches the oligosaccharide to this residue to complete that kind of glycosylation. This residue, proline, and alanine name a conserved motif found in aquaporins.
ANSWER: asparagine [or Asn; prompt on N or N-linked glycosylation by asking “what residue does that letter stand for?”; prompt on NPA motifs]
<Kane Nguyen, Biology>