CARD domains are self-activated after being bound by this structure. For 10 points each:
[10h] Name this structure formed from seven subunits of the protein Apaf-1. This structure’s function can be identified by an assay that labels 3-prime-hydroxyl termini of double-stranded DNA breaks.
ANSWER: apoptosome
[10m] CARD domains are named for the activation and recruitment of these enzymes, whose “cascade” mediates apoptosis.
ANSWER: caspases [accept caspase activation and recruitment domains; accept caspase cascade]
[10e] The six-helix structure of CARD domains are conserved in the ced-3 and ced-4 genes of this model organism. Sydney Brenner pioneered research on this nematode, which was the first multicellular organism to have its entire genome sequenced.
ANSWER: C. elegans [or Caenorhabditis elegans; prompt on Caenorhabditis]
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