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In one assay, this phenomenon is detected in a single cell if the migration pattern displays a “comet” appearance. For 10 points each:
[10m] Name this phenomenon that leads to responses such as NER or BER. This phenomenon can occur exogenously using intercalating agents such as ethidium bromide.
ANSWER: DNA damage [or DNA strand breaks; accept DNA lesions; accept single-strand breaks or SSBs; accept double-strand breaks or DSBs; accept descriptions involving DNA strands being damaged or compromised; prompt on mutagenesis or mutations; reject “DNA repair”]
[10e] The appearance of a comet in the namesake assay is attributed to broken DNA of different sizes traveling through these agarose matrices at different speeds during electrophoresis.
ANSWER: agarose gels [accept gel electrophoresis]
[10h] This is the most common kind of DNA damage detected by the comet assay, which may be formed during BER. Only the sugar backbone is retained in these single-strand breaks, missing a purine or pyrimidine.
ANSWER: AP sites [or abasic sites; accept AP endonuclease]
<Science - Biology>

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Georgia Tech BBelmont001010
Georgia Tech BBelmont001010
Georgia Tech BMTSU001010
Georgia Tech BMTSU001010
Georgia Tech BBelmont0000
Georgia Tech BBelmont0000
Georgia Tech BMTSU0000
Georgia Tech BMTSU0000
Georgia Tech AGeorgia Tech C010010
ClaremontMTSU100010
USN AWUSTL010010
Vanderbilt BVanderbilt A0000