Question
In one assay, this phenomenon is detected in a single cell if the migration pattern displays a “comet” appearance. For 10 points each:
[10m] Name this phenomenon that leads to responses such as NER or BER. This phenomenon can occur exogenously using intercalating agents such as ethidium bromide.
ANSWER: DNA damage [or DNA strand breaks; accept DNA lesions; accept single-strand breaks or SSBs; accept double-strand breaks or DSBs; accept descriptions involving DNA strands being damaged or compromised; prompt on mutagenesis or mutations; reject “DNA repair”]
[10e] The appearance of a comet in the namesake assay is attributed to broken DNA of different sizes traveling through these agarose matrices at different speeds during electrophoresis.
ANSWER: agarose gels [accept gel electrophoresis]
[10h] This is the most common kind of DNA damage detected by the comet assay, which may be formed during BER. Only the sugar backbone is retained in these single-strand breaks, missing a purine or pyrimidine.
ANSWER: AP sites [or abasic sites; accept AP endonuclease]
<Science - Biology>
Summary
| 2024 Booster Shot (Columbia) | 02/23/2024 | Y | 6 | 10.00 | 100% | 0% | 0% |
| 2024 Booster Shot (Great Lakes) | 03/09/2024 | Y | 4 | 10.00 | 75% | 25% | 0% |
| 2024 Booster Shot (Vanderbilt) | 03/02/2024 | Y | 5 | 6.00 | 40% | 20% | 0% |
| 2024 Booster Shot (Vanderbilt) | 03/02/2024 | Y | 1 | 10.00 | 0% | 0% | 100% |
| 2024 Booster Shot (WUSTL) | 03/09/2024 | Y | 3 | 10.00 | 67% | 33% | 0% |
| 2024 Booster Shot (Waterloo) | 02/23/2024 | Y | 4 | 10.00 | 75% | 25% | 0% |
Data
| Penn State B | Iowa | 0 | 0 | 0 | 0 |
| North Carolina | Iowa State | 10 | 10 | 0 | 20 |
| Ohio | Case Western | 0 | 10 | 0 | 10 |
| Stanford | WUSTL | 0 | 10 | 0 | 10 |