Question
The irreversible, covalent binding of a substrate to an active site can lead to the “suicide” form of this process. For 10 points each:
[10e] Name this process that can either occur “competitively” or “allosterically” depending on whether a molecule makes an active site inaccessible by physically blocking it or by unfavorably changing the conformation of the enzyme.
ANSWER: enzyme inhibition [or competitive inhibition; or allosteric inhibition; or suicide inhibition]
[10m] The effects of enzyme inhibition can be visualized on this plot, which converts Michaelis–Menten kinetics into a linear graph. This plot is used to quickly tell how V-max and K-sub-m change after introducing an inhibitor.
ANSWER: Lineweaver–Burk plot [or accept double reciprocal plot; prompt on Lineweaver or Burk; prompt on LB curve]
[10h] The x-intercept of a Lineweaver–Burk plot shifts to the left in the presence of competitive, but (emphasize) not non-competitive, inhibitors because the x-intercept always equals this expression of V-max and K-sub-m. You have 10 seconds.
ANSWER: negative one over K-sub-m [reject answers that include “V-max”]
<Chemistry>
Summary
| 2024 ACF Regionals @ Berkeley | 01/27/2024 | Y | 3 | 20.00 | 67% | 100% | 33% |
| 2024 ACF Regionals @ Cornell | 01/27/2024 | Y | 3 | 6.67 | 67% | 0% | 0% |
| 2024 ACF Regionals @ JMU | 01/27/2024 | Y | 10 | 6.00 | 40% | 20% | 0% |
| 2024 ACF Regionals @ Minnesota | 01/27/2024 | Y | 2 | 15.00 | 100% | 50% | 0% |
| 2024 ACF Regionals @ Nebraska | 01/27/2024 | Y | 6 | 13.33 | 100% | 33% | 0% |
| 2024 ACF Regionals @ Ohio State | 01/27/2024 | Y | 2 | 5.00 | 50% | 0% | 0% |
| 2024 ACF Regionals @ Rutgers | 01/27/2024 | Y | 5 | 20.00 | 100% | 80% | 20% |
| 2024 ACF Regionals @ Vanderbilt | 01/27/2024 | Y | 5 | 14.00 | 80% | 60% | 0% |
Data
| Columbia A | John Jay | 10 | 10 | 10 | 30 |
| Johns Hopkins | Haverford | 10 | 10 | 0 | 20 |
| Columbia B | NYU A | 10 | 10 | 0 | 20 |
| Columbia C | NYU B | 10 | 0 | 0 | 10 |
| Penn | Vassar | 10 | 10 | 0 | 20 |