Question
The irreversible, covalent binding of a substrate to an active site can lead to the “suicide” form of this process. For 10 points each:
[10e] Name this process that can either occur “competitively” or “allosterically” depending on whether a molecule makes an active site inaccessible by physically blocking it or by unfavorably changing the conformation of the enzyme.
ANSWER: enzyme inhibition [or competitive inhibition; or allosteric inhibition; or suicide inhibition]
[10m] The effects of enzyme inhibition can be visualized on this plot, which converts Michaelis–Menten kinetics into a linear graph. This plot is used to quickly tell how V-max and K-sub-m change after introducing an inhibitor.
ANSWER: Lineweaver–Burk plot [or accept double reciprocal plot; prompt on Lineweaver or Burk; prompt on LB curve]
[10h] The x-intercept of a Lineweaver–Burk plot shifts to the left in the presence of competitive, but (emphasize) not non-competitive, inhibitors because the x-intercept always equals this expression of V-max and K-sub-m. You have 10 seconds.
ANSWER: negative one over K-sub-m [reject answers that include “V-max”]
<Chemistry>
Summary
| 2024 ACF Regionals @ Berkeley | 01/27/2024 | Y | 3 | 20.00 | 67% | 100% | 33% |
| 2024 ACF Regionals @ Cornell | 01/27/2024 | Y | 3 | 6.67 | 67% | 0% | 0% |
| 2024 ACF Regionals @ JMU | 01/27/2024 | Y | 10 | 6.00 | 40% | 20% | 0% |
| 2024 ACF Regionals @ Minnesota | 01/27/2024 | Y | 2 | 15.00 | 100% | 50% | 0% |
| 2024 ACF Regionals @ Nebraska | 01/27/2024 | Y | 6 | 13.33 | 100% | 33% | 0% |
| 2024 ACF Regionals @ Ohio State | 01/27/2024 | Y | 2 | 5.00 | 50% | 0% | 0% |
| 2024 ACF Regionals @ Rutgers | 01/27/2024 | Y | 5 | 20.00 | 100% | 80% | 20% |
| 2024 ACF Regionals @ Vanderbilt | 01/27/2024 | Y | 5 | 14.00 | 80% | 60% | 0% |
Data
| Stanford B | Berkeley B | 0 | 10 | 0 | 10 |
| Berkeley C | Stanford A | 10 | 10 | 10 | 30 |
| Berkeley A | Stanford C | 10 | 10 | 0 | 20 |
| Cornell B | Binghamton | 10 | 0 | 0 | 10 |
| RIT A | Cornell A | 0 | 0 | 0 | 0 |
| Cornell C | RIT B | 10 | 0 | 0 | 10 |
| Maryland A (Grad) | GWU A (UG) | 10 | 0 | 0 | 10 |
| Liberty C (DII) | JMU B (UG) | 0 | 0 | 0 | 0 |
| Maryland B (UG) | William & Mary A (UG) | 10 | 0 | 0 | 10 |
| Maryland C (DII) | Duke A (UG) | 0 | 0 | 0 | 0 |
| UNC C (UG) | JMU A (UG) | 10 | 0 | 0 | 10 |
| UNC B (UG) | UNC D (DII) | 0 | 0 | 0 | 0 |
| Liberty A (Grad) | Virginia B (UG) | 0 | 0 | 0 | 0 |
| Virginia A (UG) | Virginia C (UG) | 0 | 10 | 0 | 10 |
| GWU B (Grad) | Roanoke College A (DII) | 0 | 0 | 0 | 0 |
| UNC A (Grad) | Liberty B (DII) | 10 | 10 | 0 | 20 |
| Arizona State | Iowa State | 10 | 10 | 0 | 20 |
| Minnesota B | Minnesota C | 10 | 0 | 0 | 10 |
| Texas A&M B | Claremont A | 10 | 0 | 0 | 10 |
| Texas A | Iowa B | 10 | 0 | 0 | 10 |
| Rice A | Appalachian State | 10 | 0 | 0 | 10 |
| UBC A | Sorbonne | 10 | 10 | 0 | 20 |
| Michigan State A | Texas A&M A | 10 | 10 | 0 | 20 |
| UW A | Iowa A | 10 | 0 | 0 | 10 |
| Ohio State A | Michigan C | 10 | 0 | 0 | 10 |
| Ohio State B | Michigan B | 0 | 0 | 0 | 0 |
| Columbia A | John Jay | 10 | 10 | 10 | 30 |
| Columbia B | NYU A | 10 | 10 | 0 | 20 |
| Columbia C | NYU B | 10 | 0 | 0 | 10 |
| Penn | Vassar | 10 | 10 | 0 | 20 |
| Johns Hopkins | Haverford | 10 | 10 | 0 | 20 |
| Geodesic | Alabama | 10 | 0 | 0 | 10 |
| Georgia Tech A | Harding | 10 | 0 | 0 | 10 |
| Kentucky | Vanderbilt B | 10 | 10 | 0 | 20 |
| MTSU | Tennessee | 0 | 10 | 0 | 10 |
| Vanderbilt A | South Carolina | 10 | 10 | 0 | 20 |