The irreversible, covalent binding of a substrate to an active site can lead to the “suicide” form of this process. For 10 points each:
[10e] Name this process that can either occur “competitively” or “allosterically” depending on whether a molecule makes an active site inaccessible by physically blocking it or by unfavorably changing the conformation of the enzyme.
ANSWER: enzyme inhibition [or competitive inhibition; or allosteric inhibition; or suicide inhibition]
[10m] The effects of enzyme inhibition can be visualized on this plot, which converts Michaelis–Menten kinetics into a linear graph. This plot is used to quickly tell how V-max and K-sub-m change after introducing an inhibitor.
ANSWER: Lineweaver–Burk plot [or accept double reciprocal plot; prompt on Lineweaver or Burk; prompt on LB curve]
[10h] The x-intercept of a Lineweaver–Burk plot shifts to the left in the presence of competitive, but (emphasize) not non-competitive, inhibitors because the x-intercept always equals this expression of V-max and K-sub-m. You have 10 seconds.
ANSWER: negative one over K-sub-m [reject answers that include “V-max”]
<Chemistry>