enzyme inhibition - 2024 ACF Regionals @ Cornell

Question

The irreversible, covalent binding of a substrate to an active site can lead to the “suicide” form of this process. For 10 points each:

[10e] Name this process that can either occur “competitively” or “allosterically” depending on whether a molecule makes an active site inaccessible by physically blocking it or by unfavorably changing the conformation of the enzyme.

ANSWER: enzyme inhibition [or competitive inhibition; or allosteric inhibition; or suicide inhibition]

[10m] The effects of enzyme inhibition can be visualized on this plot, which converts Michaelis–Menten kinetics into a linear graph. This plot is used to quickly tell how V-max and K-sub-m change after introducing an inhibitor.

ANSWER: Lineweaver–Burk plot [or accept double reciprocal plot; prompt on Lineweaver or Burk; prompt on LB curve]

[10h] The x-intercept of a Lineweaver–Burk plot shifts to the left in the presence of competitive, but (emphasize) not non-competitive, inhibitors because the x-intercept always equals this expression of V-max and K-sub-m. You have 10 seconds.

ANSWER: negative one over K-sub-m [reject answers that include “V-max”]

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Summary


2024 ACF Regionals @ Berkeley	01/27/2024	Y	3	20.00	67%	100%	33%
2024 ACF Regionals @ Cornell	01/27/2024	Y	3	6.67	67%	0%	0%
2024 ACF Regionals @ JMU	01/27/2024	Y	10	6.00	40%	20%	0%
2024 ACF Regionals @ Minnesota	01/27/2024	Y	2	15.00	100%	50%	0%
2024 ACF Regionals @ Nebraska	01/27/2024	Y	6	13.33	100%	33%	0%
2024 ACF Regionals @ Ohio State	01/27/2024	Y	2	5.00	50%	0%	0%
2024 ACF Regionals @ Rutgers	01/27/2024	Y	5	20.00	100%	80%	20%
2024 ACF Regionals @ Vanderbilt	01/27/2024	Y	5	14.00	80%	60%	0%
2023 Penn Bowl @ Waterloo	10/28/2023	N	4	17.50	100%	50%	25%
2023 Penn Bowl @ FSU	10/28/2023	N	2	20.00	100%	50%	50%
2023 Penn Bowl (Harvard)	10/21/2023	N	3	6.67	67%	0%	0%
2023 Penn Bowl (Mainsite)	10/21/2023	N	6	23.33	100%	83%	50%
2023 Penn Bowl (Norcal)	10/28/2023	N	2	20.00	100%	100%	0%
2023 Penn Bowl (South Central)	10/28/2023	N	3	6.67	67%	0%	0%
2023 Penn Bowl (UK)	10/28/2023	N	5	20.00	100%	60%	40%
2023 Penn Bowl @ UNC	10/28/2023	N	3	13.33	100%	33%	0%

Data


Cornell B	Binghamton	10	10
RIT A	Cornell A	0	0
Cornell C	RIT B	10	10