The “collateral” activation of these proteins is the core of the SHERLOCK and HOLMES methods. The SpRY (10[1])(“spry”) variant of one of these proteins is significantly more (10[1])promiscuous because it lacks key arginines at positions 1333 and 1335. A tool co-developed by Dana-Farber and the Broad (“brohd”) for designing experiments with these proteins (10[1])is called MAGeCK (10[1])(“mah-GECK”). “Dead” examples (10[1])of these proteins have been deactivated by mutations in both the HNH (10[1])and RuvC domain. (10[3])One of these proteins from S. pyogenes (“pye-OJ-uh-neez”) has been engineered to be “near-PAMless,” (10[1])expanding its range but increasing the chance of off-target cleavage. (-5[1])One-vector systems package these proteins alongside the sgRNAs (-5[1])that (-5[1])encode their target sequences. (10[1])For 10 points, name these endonucleases that are (10[1])directed by guide RNAs (10[2])produced from CRISPR (“crisper”) sequences. (10[1])■END■ (10[6]0[2])