Note to moderator: Read the answerline carefully. A clonase catalyzes this process after binding both attB (“A-T-T-B”) and attP (“A-T-T-P”) sites in the Gateway system. This process begins when the RecBCD (“rec-B-C-D”) complex encounters a Chi (“kye”) site. The Lambda red system is used in a technique that blends the word “engineering” with the name of this process, which also includes invasion and branch migration steps. After conjugative transfer, the F-factor undergoes this process at an insertion element to convert its host into an Hfr (“H-F-R”) strain. This process and non-homologous end joining are the two main pathways of double-strand break repair. The synaptonemal complex forms at chiasmata (“kye-AZ-muh-tuh”) between sister chromatids when this process occurs during prophase I (“one”) of meiosis. For 10 points, name this exchange of genetic material between two similar DNA strands. ■END■
ANSWER: homologous recombination [or word forms such as recombining; accept HR; accept genetic recombination; accept recombineering; accept crossing over; accept chromosomal crossover; prompt on insertion or integration or integrating into the chromosome by asking “by what mechanism does that integration occur?”; prompt on DNA repair or homology-directed repair or double-strand break repair or DSB repair by asking “what is the mechanism of that repair process?”]
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