Question

The “high-performance” type of this technique utilizes high pressures rather than gravity to push samples through a column. For 10 points each:
[10e] Name this class of lab techniques that use a mobile and stationary phase to separate a mixture into its components. Despite its name, it only sometimes involves color.
ANSWER: chromatography [accept high-performance liquid chromatography; prompt on HPLC or LC]
[10m] Immobilized charge groups like TEAE (“T-E-A-E”) are the stationary phase in a chromatography technique named for an “exchange” of these things. Bead-shaped resins that exchange these things are used in water purifiers.
ANSWER: ions [accept ion exchange chromatography; accept ion exchange resins; accept cations or anions]
[10h] This term refers to elutions in which the mobile phase composition is kept constant and other variables like column pressure are changed. HPLC often utilizes gradient elution rather than this type of elution.
ANSWER: isocratic elution [or isocratic flow]
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Summary

2023 ACF Winter @ Columbia11/11/2023Y721.43100%100%14%

Data

Columbia AVassar1010020
Cornell CRutgers B10101030
NYU ANYU B1010020
Penn APenn B1010020
Princeton AColumbia B1010020
Yale APrinceton B1010020
HaverfordYale C1010020