The “high-performance” type of this technique utilizes high pressures rather than gravity to push samples through a column. For 10 points each:
[10e] Name this class of lab techniques that use a mobile and stationary phase to separate a mixture into its components. Despite its name, it only sometimes involves color.
ANSWER: chromatography [accept high-performance liquid chromatography; prompt on HPLC or LC]
[10m] Immobilized charge groups like TEAE (“T-E-A-E”) are the stationary phase in a chromatography technique named for an “exchange” of these things. Bead-shaped resins that exchange these things are used in water purifiers.
ANSWER: ions [accept ion exchange chromatography; accept ion exchange resins; accept cations or anions]
[10h] This term refers to elutions in which the mobile phase composition is kept constant and other variables like column pressure are changed. HPLC often utilizes gradient elution rather than this type of elution.
ANSWER: isocratic elution [or isocratic flow]
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