Question
This substance is heated to 37 degrees Celsius before it is used for passaging. For 10 points each:
[10h] Name this substance. Examples of this substance such as DMEM (“D-M-E-M”) and RPMI (“R-P-M-I”) are usually added to quench after trypsinization (“trip-sin-eye-ZAY-shun”).
ANSWER: cell culture media [or cell culture medium; or growth media or basal media or minimal media; prompt on serum or sera by asking “what substance is that added to as a supplement?”; prompt on culture]
[10e] When grown in the right media, immortalized cell lines can perform this process indefinitely until they reach confluency in a plate. This form of cell division results in two identical diploid daughter cells.
ANSWER: mitosis [accept M phase; prompt on cell cycle or reproduction or cytokinesis; reject “meiosis”]
[10m] Cells in culture can be immortalized by expressing this enzyme, which repeatedly lengthens the ends of chromosomes to bypass the Hayflick limit on cell divisions.
ANSWER: telomerase [accept terminal transferase or TERT or hTERT]
<Biology>
Summary
| 2023 ACF Winter @ Columbia | 11/11/2023 | Y | 6 | 20.00 | 100% | 83% | 17% |
Data
| Columbia A | NYU A | 0 | 10 | 10 | 20 |
| Columbia B | Yale A | 0 | 10 | 10 | 20 |
| Rutgers A | Cornell C | 0 | 10 | 10 | 20 |
| Yale B | Haverford | 10 | 10 | 10 | 30 |
| Penn A | Princeton A | 0 | 10 | 10 | 20 |
| Princeton B | NYU B | 0 | 10 | 0 | 10 |