This substance is heated to 37 degrees Celsius before it is used for passaging. For 10 points each:
[10h] Name this substance. Examples of this substance such as DMEM (“D-M-E-M”) and RPMI (“R-P-M-I”) are usually added to quench after trypsinization (“trip-sin-eye-ZAY-shun”).
ANSWER: cell culture media [or cell culture medium; or growth media or basal media or minimal media; prompt on serum or sera by asking “what substance is that added to as a supplement?”; prompt on culture]
[10e] When grown in the right media, immortalized cell lines can perform this process indefinitely until they reach confluency in a plate. This form of cell division results in two identical diploid daughter cells.
ANSWER: mitosis [accept M phase; prompt on cell cycle or reproduction or cytokinesis; reject “meiosis”]
[10m] Cells in culture can be immortalized by expressing this enzyme, which repeatedly lengthens the ends of chromosomes to bypass the Hayflick limit on cell divisions.
ANSWER: telomerase [accept terminal transferase or TERT or hTERT]
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