Question
A form of this protein from Thermus aquaticus is used in one lab technique since it is heat-stable. For 10 points each:
[10m] Name this enzyme, used in a “chain reaction” along with dNTPs to amplify DNA sequences.
ANSWER: DNA polymerase [accept Taq polymerase; prompt on polymerase]
[10e] A heat-stable polymerase is important for the denaturation step of PCR to cleave these bonds that form double-stranded DNA. This type of bond is named after the lightest element.
ANSWER: hydrogen bonds [accept H-bonds]
[10h] In this step of PCR that comes after denaturation and before elongation, primers attach to the single-stranded DNA templates. Temperature must be closely monitored in this step for specific binding.
ANSWER: annealing
<Science - Biology>
Summary
| 2023 ACF Fall (Rutgers) | 10/14/2023 | Y | 5 | 16.00 | 100% | 40% | 20% |
Data
| Rutgers | Columbia A | 0 | 10 | 0 | 10 |
| Swarthmore A | NYU A | 10 | 10 | 10 | 30 |
| Swarthmore B | NYU B | 0 | 10 | 0 | 10 |
| University of Delaware A | Penn | 10 | 10 | 0 | 20 |
| University of Delaware B | Columbia B | 0 | 10 | 0 | 10 |