A form of this protein from Thermus aquaticus is used in one lab technique since it is heat-stable. For 10 points each:
[10m] Name this enzyme, used in a “chain reaction” along with dNTPs to amplify DNA sequences.
ANSWER: DNA polymerase [accept Taq polymerase; prompt on polymerase]
[10e] A heat-stable polymerase is important for the denaturation step of PCR to cleave these bonds that form double-stranded DNA. This type of bond is named after the lightest element.
ANSWER: hydrogen bonds [accept H-bonds]
[10h] In this step of PCR that comes after denaturation and before elongation, primers attach to the single-stranded DNA templates. Temperature must be closely monitored in this step for specific binding.
ANSWER: annealing
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