cells - 2026 ACF Regionals

Question

CASY technology is used to count these entities without the need for an electrolyte reservoir, unlike a similar apparatus invented by Walter Coulter. For 10 points each:

[10m] Name these entities that are loaded into a Neubauer counting chamber etched with five different size grids. The viability of these entities can be determined by using propidium iodide or Trypan blue staining.

ANSWER: cells [accept cytes; accept dead cells or apoptotic cells or necrotic cells; accept cell counting or cell viability assays]

[10e] Metabolic cell viability assays often quantify the amount of this molecule produced, such as after adding a firefly luciferase. This molecule is mostly generated by a namesake synthase in the inner mitochondrial membrane.

ANSWER: ATP [or adenosine triphosphate]

[10h] Another common colorimetric viability assay uses this dye, known by a three-letter acronym, which is converted into the purple compound formazan by cellular oxidoreductases.

ANSWER: MTT [or dimethylthiazol diphenyltetrazolium bromide; accept MTT assay or MTT cell viability assay; accept MTS or dimethylthiazol carboxymethoxyphenyl sulfophenyl tetrazolium; prompt on tetrazoles or tetrazolium salts]

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Summary


California (South)	Main Site	Y	4	10.00	75%	25%	0%
Canada	Main Site	Y	10	12.00	90%	30%	0%
Lower Mid-Atlantic	Main Site	Y	1	10.00	100%	0%	0%
Lower Mid-Atlantic	Main Site	Y	6	15.00	100%	50%	0%
Midwest	Main Site	Y	8	16.25	100%	63%	0%
Northeast	Main Site	Y	5	10.00	80%	20%	0%
Overflow	Main Site	Y	3	10.00	100%	0%	0%
UK	Main Site	Y	12	9.17	92%	0%	0%

Data


UC Santa Barbara	Arizona State	10	0	10
UCSD C	UC Irvine	0	10	10
UCLA A	UCSD B	0	10	10
UCSD A	UCLA B	0	10	10
Carleton University	Toronto Metropolitan	0	10	10
McMaster A	Guelph	10	10	20
Queen's	Toronto B	0	10	10
UBC A	McGill B	0	10	10
Ottawa	UBC B	0	10	10
Waterloo A	McGill A	10	10	20
Toronto G	Western B	10	10	20
Brock	Toronto D	0	0	0
Toronto E	Simon Fraser	0	10	10
Toronto F	Waterloo B	0	10	10
NC State B	Liberty B	0	10	10
Liberty C	North Carolina A	0	10	10
Liberty A	North Carolina C	0	10	10
South Carolina A	VCU	10	10	20
South Carolina B	North Carolina B	10	10	20
Virginia	Virginia Tech A	0	10	10
William and Mary	Virginia Tech B	10	10	20
Chicago B	Chicago D	10	10	20
Chicago A	Chicago C	0	10	10
Notre Dame B	Northwestern D	0	10	10
Illinois B	Purdue A	10	10	20
Indiana B	Chicago E	0	10	10
Illinois A	Illinois C	10	10	20
Indiana A	Northwestern B	10	10	20
Purdue B	Northwestern C	10	10	20
Harvard A	Bowdoin A	0	10	10
Southern Connecticut State	Bowdoin B	0	0	0
Brandeis A	Harvard B	0	10	10
MIT	Brandeis B	0	10	10
Brown	Yale	10	10	20
Iowa State	Carleton College	0	10	10
Missouri A	Georgia Tech A	0	10	10
Washington A	Claremont	0	10	10
Birmingham	LSE	0	0	0
Imperial A	Cambridge A	0	10	10
Manchester	Cambridge B	0	10	10
Cambridge C	Oxford C	0	10	10
Imperial B	Durham	0	10	10
Bristol	Edinburgh A	0	10	10
Edinburgh B	Southampton A	0	10	10
Cardiff	Imperial C	0	10	10
UCL	Montpellier	0	10	10
Oxford B	Southampton B	0	10	10
Warwick A	Oxford A	0	10	10
Warwick B	City St. George's	0	10	10