photobleaching - 2025 ACF Winter

Question

When performing time-lapse fluorescence microscopy, this phenomenon causes gradual attenuation of the signal. For 10 points each:

[10h] Name this process by which light causes irreversible chemical damage in fluorophores, rendering them unable to fluoresce. Global thresholding of signal intensity may be used to compensate for this phenomenon.

ANSWER: photobleaching [prompt on fading]

[10e] This organelle is commonly stained by the DAPI fluorophore. This organelle contains a pore complex in its envelope and contains the cell’s DNA.

ANSWER: nucleus

[10m] This protein may be added to transfection mixtures to determine cell uptake. This protein fluorophore with an emission peak of 509 nanometers was isolated from the jellyfish Aequorea victoria.

ANSWER: green fluorescent protein [or GFP]

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Summary


California	Main	Y	5	26.00	100%	100%	60%
Canada	Main	Y	6	18.33	100%	67%	17%
Florida	Main	Y	12	17.50	92%	67%	17%
Great Lakes	Main	Y	6	10.00	100%	0%	0%
Lower Mid-Atlantic	Main	Y	7	12.86	71%	57%	0%
Midwest	Main	Y	8	18.75	100%	63%	25%
North	Main	Y	11	14.55	100%	27%	18%
Northeast	Main	Y	5	16.00	100%	40%	20%
Overflow	Main	Y	4	20.00	100%	75%	25%
Upstate NY	Main	Y	8	16.25	100%	63%	0%
2025 ACF Nationals	2025-08-09	Y	14	20.00	100%	71%	29%
2024 ARCADIA at UC Berkeley	04/19/2025	Y	4	12.50	100%	25%	0%

Data


Berkeley A	Berkeley B	0	10	10	20
Stanford A	Berkeley E	10	10	10	30
Berkeley C	Stanford C	10	10	10	30
Berkeley F	UCSC	10	10	10	30
Berkeley D	Stanford B	0	10	10	20
UCSD Vices and Virtues	UCI	0	10	0	10
Claremont Scripps	UCLA A	0	10	10	20
UCLA B	Claremont Pitzer	0	10	10	20
UCSD Two Running Violet V Forms	UCLA C	10	10	10	30
UCLA D	USC	0	10	10	20
UCSB	UCSD What Hath God Wrought	0	10	0	10
Guelph A	Western Ontario	0	10	10	20
Carleton	Guelph B	0	10	10	20
Toronto A	McGill B	10	10	10	30
McMaster C	Toronto E	0	10	0	10
Ottawa	McMaster A	0	10	10	20
Queen's B	McGill C	0	10	0	10
Toronto C	Toronto H	0	10	10	20
Queen's A	Toronto I	0	10	10	20
Toronto J	Toronto G	0	0	0	0
Toronto B	Toronto K	10	10	10	30
Toronto F	Toronto Metropolitan	0	10	0	10
Toronto D	Waterloo	0	10	10	20
Melbourne A	UF D	0	10	0	10
FSU	ERAU A	0	10	0	10
UF A	FIU	0	10	0	10
UCF B	UF E	0	10	0	10
USF	ERAU B	0	10	0	10
UF B	UCF A	0	10	0	10
CWRU A	CWRU B	0	10	10	20
MSU B	CWRU C	0	0	0	0
Kenyon B	Kenyon A	0	0	0	0
MSU A	Pitt A	0	10	10	20
OSU B	OSU A	0	10	10	20
OSU D	OSU C	0	10	0	10
Pitt C	CWRU D	0	10	10	20
Clemson A	James Madison	0	10	10	20
Liberty A	Liberty C	0	10	0	10
South Carolina B	Tusculum	10	10	10	30
Tennessee A	South Carolina A	0	10	0	10
Virginia B	Tennessee B	0	10	0	10
Virginia A	Clemson B	0	10	10	20
Virginia Tech A	Roanoke	0	10	10	20
Virginia Tech B	NC State	10	10	10	30
Catholic University	George Mason A	0	10	0	10
George Washington B	George Mason C	0	10	0	10
George Washington A	Georgetown B	0	10	0	10
Johns Hopkins A	VCU A	0	10	10	20
Maryland A	George Mason B	10	10	10	30
Maryland B	Johns Hopkins B	0	10	10	20
Georgetown A	Maryland C	0	10	0	10
NYU A	Penn A	10	10	0	20
NYU B	Maryland D	0	10	0	10
Penn B	Johns Hopkins C	0	10	0	10
Notre Dame E	UMBC A	0	10	0	10
Columbia A	Lehigh B	10	10	10	30
Columbia B	Swarthmore A	0	10	10	20
Princeton A	Columbia C	0	10	0	10
Lehigh A	Rowan A	0	10	0	10
Rutgers B	Rutgers C	0	10	0	10
Missouri A	WashU B	0	10	10	20
WashU A	Missouri B	10	10	10	30
WashU C	William Woods	0	10	0	10
Truman State	WashU D	0	10	10	20
UAB A	Alabama A	0	10	10	20
Auburn A	Mississippi State A	0	10	10	20
Bruin A	Auburn B	0	10	10	20
Vanderbilt A	Georgia Tech C	0	10	0	10
Georgia Tech D	Huntsville B	0	10	0	10
Huntsville A	Sewanee A	0	10	0	10
Georgia Tech A	Vanderbilt B	0	10	10	20
Georgia Tech B	Alabama B	0	10	10	20
Imperial B	City St George's A	10	10	10	30
Birmingham A	Imperial A	0	10	10	20
Bristol A	Cambridge C	0	10	10	20
Cambridge B	Durham A	10	10	10	30
Durham B	Southampton B	0	10	0	10
Edinburgh A	Cambridge A	0	10	10	20
Imperial C	Cambridge D	10	10	10	30
Manchester A	LSE A	10	10	10	30
Manchester B	Bristol B	0	10	10	20
Oxford A	Southampton A	0	10	10	20
Sheffield B	Oxford B	0	10	10	20
Sheffield C	Lancaster A	0	10	0	10
Sheffield A	Warwick A	0	10	0	10
Warwick B	Edinburgh B	0	10	0	10
Binghamton A	RIT A	0	10	0	10
Cornell-DII	RIT B	0	10	10	20
Rochester	ESF	0	10	0	10
Syracuse	Binghamton B	0	10	0	10