photobleaching - Florida - Buzzpoints App

Question

When performing time-lapse fluorescence microscopy, this phenomenon causes gradual attenuation of the signal. For 10 points each:

[10h] Name this process by which light causes irreversible chemical damage in fluorophores, rendering them unable to fluoresce. Global thresholding of signal intensity may be used to compensate for this phenomenon.

ANSWER: photobleaching [prompt on fading]

[10e] This organelle is commonly stained by the DAPI fluorophore. This organelle contains a pore complex in its envelope and contains the cell’s DNA.

ANSWER: nucleus

[10m] This protein may be added to transfection mixtures to determine cell uptake. This protein fluorophore with an emission peak of 509 nanometers was isolated from the jellyfish Aequorea victoria.

ANSWER: green fluorescent protein [or GFP]

Back to bonuses

Summary


Northern California	Main	Y	5	26.00	100%	100%	60%
Southern California	Main	Y	6	18.33	100%	67%	17%
Eastern Canada	Main	Y	12	17.50	92%	67%	17%
Florida	Main	Y	6	10.00	100%	0%	0%
Great Lakes	Main	Y	7	12.86	71%	57%	0%
Lower Mid-Atlantic	Main	Y	8	18.75	100%	63%	25%
Upper Mid-Atlantic (1)	Main	Y	11	14.55	100%	27%	18%
Upper Mid-Atlantic (2)	Main	Y	5	16.00	100%	40%	20%
Lower Midwest	Main	Y	4	20.00	100%	75%	25%
Southeast	Main	Y	8	16.25	100%	63%	0%
UK	Main	Y	14	20.00	100%	71%	29%
Upstate NY	Main	Y	4	12.50	100%	25%	0%

Data


FSU	ERAU A	10	10
Melbourne A	UF D	10	10
UF B	UCF A	10	10
UF A	FIU	10	10
UCF B	UF E	10	10
USF	ERAU B	10	10