CRISPR-Cas9 - 2025 ACF Winter

Question

HNH and RuvC are key endonuclease domains in an enzyme that is used in this pathway. For 10 points each:

[10m] Name this gene editing pathway originally found in bacteria that often uses an RNA-guided endonuclease to induce edits in a target region of DNA.

ANSWER: CRISPR-Cas9 [or clustered regularly interspaced short palindromic repeats]

[10h] This 2-6 base pair DNA sequence downstream of the target DNA sequence must be recognized by the endonuclease for successful DNA binding in CRISPR editing. For the endonuclease SpCas9, this sequence is NGG.

ANSWER: PAM [or protospacer adjacent motif]

[10e] CRISPR can be used to create knockout mutants in this organism that includes the BALB/c albino strain. These rodents smaller than rats are used as common model organisms in labs.

ANSWER: laboratory mice [or laboratory mouse; accept Mus musculus]

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Summary


California	Main	Y	5	22.00	100%	80%	40%
Canada	Main	Y	6	16.67	100%	67%	0%
Great Lakes	Main	Y	3	20.00	100%	100%	0%
Lower Mid-Atlantic	Main	Y	6	18.33	100%	83%	0%
Midwest	Main	Y	10	18.00	100%	70%	10%
North	Main	Y	10	20.00	100%	80%	20%
Northeast	Main	Y	4	17.50	100%	75%	0%
Pacific Northwest	Main	Y	9	21.11	100%	89%	22%
Pacific Northwest	Main	Y	1	20.00	100%	100%	0%
South Central	Main	Y	5	18.00	100%	80%	0%
Southeast	Main	Y	10	19.00	100%	60%	30%
UK	Main	Y	4	15.00	100%	50%	0%
Upper Mid-Atlantic	Main	Y	8	20.00	100%	63%	38%
Upstate NY	Main	Y	7	15.71	100%	57%	0%
2025 ACF Nationals	2025-08-09	Y	11	19.09	91%	91%	9%
2024 ARCADIA at UC Berkeley	04/19/2025	Y	4	20.00	100%	100%	0%

Data


Berkeley A	Berkeley D	0	0	10	10
Berkeley F	Berkeley C	10	10	10	30
Stanford A	Berkeley B	10	10	10	30
UCSC	Stanford B	10	0	10	20
Claremont Scripps	UCSD Two Running Violet V Forms	10	0	10	20
UCLA D	UCI	10	0	10	20
UCLA A	UCSB	0	0	10	10
UCLA B	UCSD Vices and Virtues	10	0	10	20
UCSD What Hath God Wrought	UCLA C	10	0	10	20
Claremont Pitzer	USC	0	0	10	10
Stanford C	Berkeley E	10	0	10	20
UF E	Melbourne B	10	0	10	20
UF C	ERAU A	10	0	10	20
USF	UF A	10	0	10	20
CWRU A	MSU A	10	0	10	20
OSU D	CWRU B	0	0	10	10
Kenyon B	CWRU D	10	0	10	20
MSU B	OSU B	10	0	10	20
OSU C	OSU A	10	0	10	20
Pitt B	Pitt C	10	0	10	20
Tennessee A	Clemson B	10	0	10	20
Liberty A	Tennessee B	10	0	10	20
North Carolina C	Liberty C	0	0	10	10
James Madison	North Carolina B	0	0	10	10
Virginia B	Roanoke	10	0	10	20
North Carolina A	South Carolina B	0	0	10	10
South Carolina A	Tusculum	10	0	10	20
Virginia A	NC State	10	0	10	20
Virginia Tech A	Clemson A	10	0	10	20
Virginia Tech B	Liberty B	10	10	10	30
Georgetown A	George Mason B	10	0	10	20
Maryland C	Georgetown B	10	0	10	20
Johns Hopkins C	George Washington A	10	0	10	20
Maryland A	George Mason C	10	0	10	20
Penn A	Maryland B	10	10	10	30
NYU A	Catholic University	10	0	10	20
George Washington B	NYU B	0	0	10	10
Johns Hopkins A	Notre Dame E	10	10	10	30
Maryland D	Penn B	0	0	10	10
VCU A	UMBC A	10	0	10	20
Columbia A	Princeton A	10	0	10	20
Swarthmore A	Columbia C	10	0	10	20
Rowan A	Lehigh B	10	0	10	20
Rutgers B	Columbia B	0	0	10	10
Michigan A	Chicago D	10	0	10	20
Indiana A	Purdue C	10	0	10	20
Illinois A	Indiana B	10	0	10	20
Louisville B	Michigan B	10	10	10	30
Chicago A	Michigan D	0	10	10	20
Notre Dame B	UIC	10	0	10	20
Notre Dame D	Kentucky	10	0	10	20
Purdue A	Illinois B	10	0	10	20
Notre Dame A	Purdue B	10	0	10	20
Chicago C	Notre Dame C	10	0	10	20
Carleton	Minnesota C	10	0	10	20
Minnesota A	Macalester A	0	0	10	10
Macalester B	Iowa State	10	0	10	20
Nebraska	St. Thomas	10	0	10	20
Minnesota B	Wisconsin	10	0	10	20
BC A	Brown A	0	0	10	10
BU	Brandeis Orlando	10	0	10	20
Brandeis Clemenceau	Yale A	10	0	10	20
Amherst A	Brown C	10	0	10	20
MIT A	Brown B	10	10	10	30
MIT B	Dartmouth	0	0	10	10
Amherst B	Tufts A	0	0	10	10
Brandeis Wilson	Tufts B	0	0	10	10
Williams	Bowdoin A	10	10	10	30
Harvard	Yale B	10	10	10	30
Washington B	British Columbia A	0	0	10	10
Washington A	British Columbia D	10	0	10	20
Simon Fraser	British Columbia C	0	0	10	10
Alberta	British Columbia B	10	0	10	20
Alberta B	UTD A	0	0	10	10
Arizona State A	Northwestern B	10	10	10	30
Northwestern A	Arizona State B	10	10	10	30
Ohio	Tulsa	0	0	10	10
Iowa	Rose State	10	0	10	20
Texas A	McGill D	10	10	10	30
Texas B	Northwestern C	0	0	10	10
UCO	UTD B	10	0	10	20
Georgia Tech A	Alabama A	10	0	10	20
Georgia Tech B	Auburn A	10	0	10	20
Georgia Tech C	Alabama B	0	0	10	10
Vanderbilt B	Georgia Tech D	10	0	10	20
Vanderbilt A	Huntsville A	10	0	10	20
Bruin A	Huntsville B	0	0	10	10
Auburn B	UAB A	0	0	10	10
LSE A	Cambridge C	10	0	10	20
Sheffield B	Birmingham A	10	0	10	20
Imperial A	Cambridge B	10	0	10	20
Durham B	Sheffield C	10	0	10	20
Warwick B	Edinburgh B	10	0	10	20
Edinburgh A	Imperial B	0	0	0	0
Bristol A	LSE B	10	0	10	20
Bristol B	Lancaster A	10	0	10	20
Manchester A	Southampton A	10	0	10	20
Cambridge A	Manchester B	10	10	10	30
Oxford A	Imperial C	10	0	10	20
Cornell-DII	ESF	10	0	10	20
Rochester	RIT A	10	0	10	20
RIT B	Binghamton B	10	0	10	20
Binghamton A	Syracuse	10	0	10	20