CRISPR-Cas9 - Florida - Buzzpoints App

Question

HNH and RuvC are key endonuclease domains in an enzyme that is used in this pathway. For 10 points each:

[10m] Name this gene editing pathway originally found in bacteria that often uses an RNA-guided endonuclease to induce edits in a target region of DNA.

ANSWER: CRISPR-Cas9 [or clustered regularly interspaced short palindromic repeats]

[10h] This 2-6 base pair DNA sequence downstream of the target DNA sequence must be recognized by the endonuclease for successful DNA binding in CRISPR editing. For the endonuclease SpCas9, this sequence is NGG.

ANSWER: PAM [or protospacer adjacent motif]

[10e] CRISPR can be used to create knockout mutants in this organism that includes the BALB/c albino strain. These rodents smaller than rats are used as common model organisms in labs.

ANSWER: laboratory mice [or laboratory mouse; accept Mus musculus]

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Summary


Northern California	Main	Y	5	22.00	100%	80%	40%
Southern California	Main	Y	6	16.67	100%	67%	0%
Florida	Main	Y	3	20.00	100%	100%	0%
Great Lakes	Main	Y	6	18.33	100%	83%	0%
Lower Mid-Atlantic	Main	Y	10	18.00	100%	70%	10%
Upper Mid-Atlantic (1)	Main	Y	10	20.00	100%	80%	20%
Upper Mid-Atlantic (2)	Main	Y	4	17.50	100%	75%	0%
Upper Midwest	Main	Y	9	21.11	100%	89%	22%
Upper Midwest	Main	Y	1	20.00	100%	100%	0%
North	Main	Y	5	18.00	100%	80%	0%
Northeast	Main	Y	10	19.00	100%	60%	30%
Northwest	Main	Y	4	15.00	100%	50%	0%
South Central	Main	Y	8	20.00	100%	63%	38%
Southeast	Main	Y	7	15.71	100%	57%	0%
UK	Main	Y	11	19.09	91%	91%	9%
Upstate NY	Main	Y	4	20.00	100%	100%	0%

Data


UF E	Melbourne B	10	10	20
UF C	ERAU A	10	10	20
USF	UF A	10	10	20