In the Providence mutant of this protein, its lysine EF6 residue is replaced by an asparagine residue, causing its affinity for 2,3-diphosphoglycerate (“two-three-di-phospho-glycerate”) to decrease. For 10 points each:
[10e] Name this iron-containing protein found in red blood cells that transports oxygen through the human body.
ANSWER: hemoglobin [accept Hb or Hgb; prompt on heme]
[10m] Oxygen’s binding affinity to hemoglobin is affected by pH and this dependent variable, according to the Bohr effect. A higher value of this variable leads to more bicarbonate formation, in turn changing the blood pH.
ANSWER: carbon dioxide concentration [accept CO2 concentration]
[10h] Iron centers in heme are stabilized by ligands of this compound, which bind the heme to the rest of hemoglobin. The amino acid with this heterocyclic compound as a side chain is positively charged at physiological pH.
ANSWER: imidazoles (“ih-MIDE-uh-zoles”) [accept 1,3-diazoles; prompt on histidine or His]
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