The 6-carboxy modified derivative of this compound is microinjected into liposomes to track drug delivery. For 10 points each:
[10h] Name this xanthine-based compound, whose isothiocyanate-functionalized derivative is commonly used to label cells green in flow cytometry. Brominating this compound generates the eosin Y stain used in Pap smear tests.
ANSWER: fluorescein [accept 6-carboxyfluorescein or 6-FAM; accept fluorescein isothiocyanate or FITC]
[10e] Fluorescein derivatives like Alexa Fluor 488 are widely used in super-resolution microscopy to study systems limited by this phenomenon, which occurs when X-rays interact with crystalline structures according to Bragg’s law.
ANSWER: diffraction [accept diffraction limit or diffraction-limited system; accept Abbe diffraction; accept X-ray diffraction; prompt on interference]
[10m] Alexa dyes are also used in fluorescence recovery after photobleaching to measure the rate of this process for cell membranes. Coefficients for this process are generally lower in lipid rafts due to better lipid packing.
ANSWER: diffusion [accept diffusivity; accept diffusion coefficient or D; accept Brownian motion; prompt on lateral motion or movement; prompt on mass transport]
<UCF A, Chemistry>