Description acceptable. An ultra-stable superfolder GFP was created to tolerate procedures that confer this property, which often require weeks-long immersions in Triton X-100. The Scale and CUBIC protocols for producing this property may cause sample expansion, which can be controlled by embedding in a hydrogel. An alleged consequence of the Kramers–Kronig relations is that topically applying a strong absorber like tartrazine can cause this property in a sample. Methods in the DISCO family give 3D samples this property, allowing (*) microtoming to be replaced with optical sectioning by light sheets. Delipidation and fluid displacement by benzyl ether gives a fixed and stained tissue sample this property in the process of “clearing.” For 10 points, what desirable property is achieved by refractive index matching that allows imaging tissues’ internal structures? ■END■
ANSWER: tissue transparency [or making tissues transparent; accept tissue clearing or making tissues clear until “clearing” is read; prompt on making tissues translucent; prompt on bulk, thick, or three-dimensional until “3D” is read; prompt on prepared, stained, fixed, or mounted tissue samples until “fixed” is read] (The CLARITY procedure uses a hydrogel.)
<S, Biology>
= Average correct buzz position