In one assay, a “22F” plasmid encodes a circularly permuted form of this protein that uses cAMP to find its active conformation. A synthetic form of this protein includes a complementary Large BiT and Small BiT that bind each other when their target proteins are together. This protein’s main substrate is regenerated in vivo by hydrolyzing its product to form CHBT, which then reacts with cysteine. Promega kits such as NanoBiT use commercial forms of this enzyme, including one from the shrimp (15[1])(*) Oplophorus gracilirostris. A magnesium- and ATP-independent (10[1])form of this enzyme (10[1])oxidizes coelenterazine (10[1])in Renilla and yields the more stable of two signals in certain “dual” assays. The genus Photinus uses this enzyme to create flash (10[1])patterns (10[1])in its lantern organ. For 10 (10[1])points, (10[1])what enzyme (10[1])in bioluminescent (10[2])reporter assays causes (-5[1])fireflies to glow? ■END■ (0[1])