In the lambda phage, the N protein binds the boxB motif to create a loop that inhibits this process. For 10 points each:
[10m] Name this process, which can occur via rho-dependent or rho-independent mechanisms. The DNA sequence that controls this process is found directly downstream of the 3’ (“3-prime”) UTR.
ANSWER: transcription termination [prompt on transcription]
[10h] In this model of transcription termination, exonucleases like XRN2 or Rat1 degrade residual mRNA bound to the DNA template until they collide with RNA polymerase II. It is contrasted with the allosteric model.
ANSWER: torpedo model
[10e] Eukaryotic terminators generally contain a motif that signals the addition of this post-transcriptional modification. This adenine-rich sequence protects the 3’ (“3-prime”) end of eukaryotic mRNA.
ANSWER: poly(A) tail [accept polyadenylation; prompt on poly(A) or tail]
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