chromatography - 2024 PLAYTIME @ Harvard

Question

Oligonucleotide synthesis products can sometimes be identified by observing the highest peak created following application of this technique’s “high-performance liquid” form. For 10 points each:

[10e] Name this lab technique that generally separates a mixture by passing a mobile phase over a stationary phase.

ANSWER: chromatography [accept high performance liquid chromatography or high pressure liquid chromatography; prompt on HPLC]

[10h] A structure with this name that is formed due to an unconventional oxygen-carbon bond is used to “lock” ribose in the 3’ (“three-prime”) endo conformation for solid-state oligonucleotide synthesis. Ligands that create structures with this name are contrasted with terminal ligands.

ANSWER: bridges [accept bridged nucleic acids; accept bridging ligands]

[10m] Splint oligonucleotides and synthetic oligonucleotide probes are sometimes used in a “high throughput” form of this technique named for nanoballs. A different type of this technique that uses overlapping results to reconstruct data gets its name from the relationship of the results to quasi-random bullet holes.

ANSWER: DNA sequencing [accept DNA nanoball sequencing; accept high throughput sequencing; accept shotgun sequencing]

<SM, Chemistry>

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