Question
In one technique, production of these molecules begins with the fusion of antigen-exposed B-cells to HGPRT negative, immortal myeloma cells. For 10 points each:
[10e] Name these molecules that are collected and “humanized” prior to therapeutic use. These molecules are produced from identical clones of a single parent cell.
ANSWER: monoclonal antibodies [or mAbs; prompt on antibodies or immunoglobulins]
[10h] During monoclonal antibody production, hybridoma cells are cultured in this three-component selection medium that blocks DNA de novo synthesis to restrict the growth of unfused cells.
ANSWER: HAT medium [or hypoxanthine-aminopterin-thymidine medium]
[10m] The aminopterin in HAT medium disrupts DNA synthesis by blocking the reductase activity of an enzyme that acts on this metabolite. That enzyme, which is targeted by the chemotherapy drug methotrexate, converts this compound’s less active “di-hydro” form into its more active “tetra-hydro” form.
ANSWER: folate [or folic acid or vitamin B9 or folacin; accept dihydrofolate reductase or tetrahydrofolate; prompt on DHFR or THF]
<RH, Biology>
Summary
| 2024 Chicago Open | 07/28/2024 | Y | 8 | 15.00 | 63% | 50% | 38% |
Data
| A Nervy Liberal Nanny Hence | SAYYID QUTB, DRIVING THE LONGEST CAR YOU’VE EVER SEEN THROUGH TAHRIR SQUARE | 10 | 0 | 10 | 20 |
| The Empire Bikes Back | Are You There, Chat? It's Me, Margaret | 0 | 0 | 0 | 0 |
| Charlotte's Moderately Successful Team | Galvanized Square Steen and Eco-Friendly Wood Vermeer | 10 | 10 | 10 | 30 |
| Lying on a Mattress at Lou’s Apartment in Pilsen, Chicago | I’ll take a quiet 10 / A handshake of CO (Chicago Open) | 0 | 0 | 0 | 0 |
| genAI this, genAI that, have you found your 真爱 yet | LMM's LLM MLM | 10 | 10 | 10 | 30 |
| Berkeley Mafia and the Chicago Boy | NJTRANSit (and anwen, I guess) | 0 | 0 | 0 | 0 |
| Punished "Venom" BHSU | The Jeam Team | 10 | 10 | 10 | 30 |
| The Oldest Men in the World | BHSU B | 10 | 0 | 0 | 10 |