Question
The simplest version of this technique consists of two flasks, a chemostat and a lagoon, connected to pumps that deliver fresh media and move excess cells to a waste container. For 10 points each:
[10h] Name this protein evolution technique in which fresh, host E. coli from the chemostat are passed to the lagoon, which contains infected E. coli, to allow genes to be passed through many generations of infectious progeny.
ANSWER: PACE [or phage-assisted continuous evolution]
[10m] PACE was developed using the M13 bacteriophage, a member of the Inoviridae family, which collectively have this shape. The f1 and fd phages used by Smith and Winter for their pioneering phage display experiments also have this shape.
ANSWER: filamentous [prompt on worm-like or tailed bacteriophages]
[10e] CPR, an emulsion-based protein evolution technique, selects for any alteration that results in an increased production of this heat-stable polymerase, since this critical polymerase for PCR mediates its final step.
ANSWER: Taq polymerase
<RH, Biology>
Summary
| 2024 Chicago Open | 07/28/2024 | Y | 15 | 8.67 | 87% | 0% | 0% |